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PMID: 2997745 Published · ppublish English Journal Article

The nucleotide sequence of the yeast MEL1 gene.

Nucleic acids research ·Vol. 13 ·No. 20 ·1985-10-25 ·Pages 7257-68

Liljeström PL

Abstract

The complete nucleotide sequence of the MEL1 gene of the yeast, Saccharomyces cerevisiae, encoding alpha-galactosidase was determined. The nucleotide sequence contains an open reading frame of 1413 bp encoding a protein of 471 amino acids. Comparison with the known N-terminal amino acid sequence of the mature secreted protein indicated that alpha-galactosidase is synthesized as a precursor with an N-terminal signal sequence of 18 amino acids. The general features of this signal peptide resemble those of other yeast signal peptides. Molecular weight of the mature alpha-galactosidase polypeptide deduced from the nucleotide sequence is 50.049 kd. The 5' regulatory region has sequences in common with other yeast genes regulated by the GAL4-protein.

MeSH Terms
Amino Acid Sequence Base Sequence Chromosome Mapping Codon DNA, Fungal/genetics Endonucleases Galactosidases/genetics Gene Expression Regulation Genes Genes, Fungal Molecular Weight Protein Conformation Protein Sorting Signals Saccharomyces cerevisiae/genetics Single-Strand Specific DNA and RNA Endonucleases Solubility Transcription Factors/genetics alpha-Galactosidase/genetics
Chemicals
Codon DNA, Fungal Protein Sorting Signals Transcription Factors Endonucleases Single-Strand Specific DNA and RNA Endonucleases Galactosidases alpha-Galactosidase
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Liljeström P L
References (43)
43 references, click to expand
  1. The sequence 5'-AAUAAA-3'forms parts of the recognition site for polyadenylation of late SV40 mRNAs.
    Cell. 1981 Apr;24(1):251-60 PMID: 6113054
  2. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  3. The organization and transcription of the galactose gene cluster of Saccharomyces.
    J Mol Biol. 1981 Oct 25;152(2):285-315 PMID: 6276569
  4. The primary structure of the Saccharomyces cerevisiae gene for alcohol dehydrogenase.
    J Biol Chem. 1982 Mar 25;257(6):3018-25 PMID: 6277922
  5. Codon selection in yeast.
    J Biol Chem. 1982 Mar 25;257(6):3026-31 PMID: 7037777
  6. DNA sequence required for efficient transcription termination in yeast.
    Cell. 1982 Mar;28(3):563-73 PMID: 6280875
  7. Conservation of high efficiency promoter sequences in Saccharomyces cerevisiae.
    Nucleic Acids Res. 1982 Apr 24;10(8):2625-37 PMID: 6281737
  8. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  9. Structure of a yeast pheromone gene (MF alpha): a putative alpha-factor precursor contains four tandem copies of mature alpha-factor.
    Cell. 1982 Oct;30(3):933-43 PMID: 6754095
  10. Mechanism of incorporation of cell envelope proteins in Escherichia coli.
    Annu Rev Microbiol. 1982;36:435-65 PMID: 6756294
  11. Isolation and preliminary characterization of the GAL4 gene, a positive regulator of transcription in yeast.
    Proc Natl Acad Sci U S A. 1982 Nov;79(22):6827-31 PMID: 6294656
  12. Nucleotide sequence of the yeast SUC2 gene for invertase.
    Nucleic Acids Res. 1983 Mar 25;11(6):1943-54 PMID: 6300785
  13. Saccharomyces cerevisiae contains two discrete genes coding for the alpha-factor pheromone.
    Nucleic Acids Res. 1983 Jun 25;11(12):4049-63 PMID: 6306574
  14. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  15. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.
    Nucleic Acids Res. 1984 Jan 25;12(2):857-72 PMID: 6694911
  16. Sequence of the preprotoxin dsRNA gene of type I killer yeast: multiple processing events produce a two-component toxin.
    Cell. 1984 Mar;36(3):741-51 PMID: 6697395
  17. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis.
    Gene. 1983 Dec;26(1):101-6 PMID: 6323249
  18. Molecular cloning and characterization of the RAD1 gene of Saccharomyces cerevisiae.
    Gene. 1983 Dec;26(2-3):119-26 PMID: 6368317
  19. Compilation of published signal sequences.
    Nucleic Acids Res. 1984 Jul 11;12(13):5145-64 PMID: 6379599
  20. Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae.
    Mol Cell Biol. 1984 Aug;4(8):1440-8 PMID: 6092912
  21. Structural analysis of the two tandemly repeated acid phosphatase genes in yeast.
    Nucleic Acids Res. 1984 Oct 25;12(20):7721-39 PMID: 6093051
  22. Analysis of membrane and surface protein sequences with the hydrophobic moment plot.
    J Mol Biol. 1984 Oct 15;179(1):125-42 PMID: 6502707
  23. Regulation of basal and induced levels of the MEL1 transcript in Saccharomyces cerevisiae.
    Mol Cell Biol. 1984 Jul;4(7):1238-45 PMID: 6209559
  24. Saccharomyces cerevisiae GAL1-GAL10 divergent promoter region: location and function of the upstream activating sequence UASG.
    Mol Cell Biol. 1984 Nov;4(11):2467-78 PMID: 6392852
  25. Nucleotide sequence of the extracellular glucoamylase gene STA1 in the yeast Saccharomyces diastaticus.
    J Bacteriol. 1985 Feb;161(2):567-73 PMID: 3918017
  26. Specific DNA binding of GAL4, a positive regulatory protein of yeast.
    Cell. 1985 Apr;40(4):767-74 PMID: 3886158
  27. Regulation of genes controlling synthesis of the galactose pathway enzymes in yeast.
    Genetics. 1966 Sep;54(3):911-6 PMID: 5970626
  28. Induction of galactokinase in Saccharomyces cerevisiae: kinetics of induction and glucose effects.
    J Bacteriol. 1972 Aug;111(2):308-15 PMID: 4559724
  29. Studies on the positive regulatory gene, GAL4, in regulation of galactose catabolic enzymes in Saccharomyces cerevisiae.
    Mol Gen Genet. 1974;135(3):203-12 PMID: 4376212
  30. Genetic co-regulation of galactose and melibiose utilization in Saccharomyces.
    J Bacteriol. 1976 Jan;125(1):33-41 PMID: 1245460
  31. Interaction of super-repressible and dominant constitutive mutations for the synthesis of galactose pathway enzymes in Saccharomyces cerevisiae.
    Mol Gen Genet. 1977 Apr 29;152(3):137-44 PMID: 327270
  32. alpha-Galactosidase from Saccharomyces carlsbergensis. Cellular localization, and purification of the external enzyme.
    Eur J Biochem. 1977 Jul 15;77(2):375-82 PMID: 891541
  33. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  34. Genetic control of galactokinase synthesis in Saccharomyces cerevisiae: evidence for constitutive expression of the positive regulatory gene gal4.
    J Bacteriol. 1978 May;134(2):446-57 PMID: 207666
  35. Regulation of the galactose pathway in Saccharomyces cerevisiae: induction of uridyl transferase mRNA and dependency on GAL4 gene function.
    Proc Natl Acad Sci U S A. 1978 Jun;75(6):2878-82 PMID: 351620
  36. Prediction of the secondary structure of proteins from their amino acid sequence.
    Adv Enzymol Relat Areas Mol Biol. 1978;47:45-148 PMID: 364941
  37. alpha-Galactosidase (melibiase) from Saccharomyces carlsbergensis: structrual and kinetic properties.
    Arch Biochem Biophys. 1978 Nov;191(1):316-24 PMID: 216315
  38. The primary structure of a glyceraldehyde-3-phosphate dehydrogenase gene from Saccharomyces cerevisiae.
    J Biol Chem. 1979 Oct 10;254(19):9839-45 PMID: 385592
  39. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  40. Structural comparison of two nontandemly repeated yeast glyceraldehyde-3-phosphate dehydrogenase genes.
    J Biol Chem. 1980 Mar 25;255(6):2596-605 PMID: 6244283
  41. Function of positive regulatory gene gal4 in the synthesis of galactose pathway enzymes in Saccharomyces cerevisiae: evidence that the GAL81 region codes for part of the gal4 protein.
    J Bacteriol. 1980 Feb;141(2):508-27 PMID: 6988385
  42. Transcription maps of adenovirus.
    Methods Enzymol. 1980;65(1):750-68 PMID: 6246372
  43. Organization and expression of eucaryotic split genes coding for proteins.
    Annu Rev Biochem. 1981;50:349-83 PMID: 6791577
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.20.7257
Published
1985-10-25
Pages
7257-68
Language
English
Region
England
NLM ID
0411011
PMCID
PMC322042
Subset
IM
Databases
GENBANK
X03102
External Links
PubMed source DOI: 10.1093/nar/13.20.7257 PMC Full Text View journal details
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