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PMID: 2999451 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Structure and evolution of two related transcription units of Epstein-Barr virus carrying small tandem repeats.

Journal of virology ·Vol. 56 ·No. 3 ·1985-12-00 ·Pages 987-95

Laux G, Freese UK, Bornkamm GW

Abstract

Two regions of the Epstein-Barr virus (EBV) genome carrying partially homologous clusters of short tandem repeats (NotI and PstI repeats) flanked by 1044 and 1045 base pairs with almost complete homology (DL and DR, left and right duplication, respectively) were most abundantly transcribed into poly(A)+ mRNA after induction with the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate. The nucleotide sequence of both repeat clusters and the conserved upstream regulatory sequences from the M-ABA EBV strain are presented. Nearly the whole part of the sequences coding for the RNAs is covered by the NotI and PstI repeats, respectively. The regulatory sequences for these genes are located in the homologous regions of 1044 and 1045 base pairs (DL and DR, respectively). A CAAT box, a TATA box, and other herpes simplex virus-like elements were identified for both transcription units. The initiation points and the 3' ends of both inducible RNAs were mapped by S1 nuclease analysis. Both genes have open reading frames and may potentially code for proteins with repetitive amino acid compositions. The structure of these two inducible EBV genes is discussed, and an evolutionary model is proposed for the generation of gene duplication in the M-ABA strain of EBV.

MeSH Terms
Biological Evolution Chromosome Mapping DNA Restriction Enzymes Gene Expression Regulation Genes, Viral Herpesvirus 4, Human/genetics Poly A/genetics Promoter Regions, Genetic RNA, Viral/genetics Repetitive Sequences, Nucleic Acid Transcription, Genetic Viral Proteins/genetics
Chemicals
RNA, Viral Viral Proteins Poly A DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Laux G
Freese U K
Bornkamm G W
References (41)
41 references, click to expand
  1. DNA of Epstein-Barr virus VIII: B95-8, the previous prototype, is an unusual deletion derivative.
    Cell. 1980 Nov;22(1 Pt 1):257-67 PMID: 6253079
  2. Comparison of Epstein-Barr virus strains of different origin by analysis of the viral DNAs.
    J Virol. 1980 Sep;35(3):603-18 PMID: 6252328
  3. The collagen gene: evidence for its evolutinary assembly by amplification of a DNA segment containing an exon of 54 bp.
    Cell. 1980 Dec;22(3):887-92 PMID: 7460017
  4. The primary structure of a plant storage protein: zein.
    Nucleic Acids Res. 1981 Oct 10;9(19):5163-74 PMID: 6895552
  5. A flexible new computer program for handling DNA sequence data.
    Nucleic Acids Res. 1982 Jan 11;10(1):229-36 PMID: 6278406
  6. Organization of the Epstein-Barr virus DNA molecule. II. Fine mapping of the boundaries of the internal repeat cluster of B95-8 and identification of additional small tandem repeats adjacent to the HR-1 deletion.
    J Virol. 1982 Jul;43(1):201-12 PMID: 6286998
  7. Mapping of polypeptides encoded by the Epstein-Barr virus genome in productive infection.
    Proc Natl Acad Sci U S A. 1982 Sep;79(18):5698-702 PMID: 6291060
  8. A hierarchic arrangement of the repetitive sequences in the Balbiani ring 2 gene of Chironomus tentans.
    Cell. 1982 Sep;30(2):579-87 PMID: 6291776
  9. Comparisons of the complete sequences of two collagen genes from Caenorhabditis elegans.
    Cell. 1982 Sep;30(2):599-606 PMID: 7139711
  10. Identification and nucleotide sequences of two similar tandem direct repeats in Epstein-Barr virus DNA.
    J Virol. 1982 Dec;44(3):823-33 PMID: 6294332
  11. Structural features of the herpes simplex virus alpha gene 4, 0, and 27 promoter-regulatory sequences which confer alpha regulation on chimeric thymidine kinase genes.
    J Virol. 1982 Dec;44(3):939-49 PMID: 6294341
  12. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  13. Homologous upstream sequences near Epstein-Barr virus promoters.
    Proc Natl Acad Sci U S A. 1983 Mar;80(6):1565-9 PMID: 6300857
  14. Clustered repeat sequences in the genome of Epstein Barr virus.
    Nucleic Acids Res. 1983 Jun 25;11(12):3919-37 PMID: 6306567
  15. Organization of the Epstein-Barr virus DNA molecule. III. Location of the P3HR-1 deletion junction and characterization of the NotI repeat units that form part of the template for an abundant 12-O-tetradecanoylphorbol-13-acetate-induced mRNA transcript.
    J Virol. 1983 Oct;48(1):135-48 PMID: 6310141
  16. Structure of the plasmodium knowlesi gene coding for the circumsporozoite protein.
    Cell. 1983 Oct;34(3):815-22 PMID: 6313209
  17. High-resolution characterization of herpes simplex virus type 1 transcripts encoding alkaline exonuclease and a 50,000-dalton protein tentatively identified as a capsid protein.
    J Virol. 1983 Dec;48(3):591-603 PMID: 6313961
  18. Two distant clusters of partially homologous small repeats of Epstein-Barr virus are transcribed upon induction of an abortive or lytic cycle of the virus.
    J Virol. 1983 Dec;48(3):731-43 PMID: 6313969
  19. DNA sequence homology between two co-linear loci on the HSV genome which have different transforming abilities.
    EMBO J. 1983;2(11):1953-61 PMID: 6315408
  20. The end of the message and beyond.
    Nature. 1984 Feb 2-8;307(5950):412-3 PMID: 6694735
  21. Simplified computer programs for search of homology within nucleotide sequences.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):193-201 PMID: 6546417
  22. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.
    Nucleic Acids Res. 1984 Jan 25;12(2):857-72 PMID: 6694911
  23. Replication origins and a sequence involved in coordinate induction of the immediate-early gene family are conserved in an intergenic region of herpes simplex virus.
    Nucleic Acids Res. 1984 Feb 24;12(4):2061-79 PMID: 6322134
  24. Two distant regions of the Epstein-Barr virus genome with sequence homologies have the same orientation and involve small tandem repeats.
    EMBO J. 1982;1(1):21-6 PMID: 6325150
  25. Initiation of translation at internal AUG codons in mammalian cells.
    Nature. 1984 May 3-9;309(5963):82-5 PMID: 6717585
  26. Selection of initiation sites by eucaryotic ribosomes: effect of inserting AUG triplets upstream from the coding sequence for preproinsulin.
    Nucleic Acids Res. 1984 May 11;12(9):3873-93 PMID: 6328442
  27. A complete set of overlapping cosmid clones of M-ABA virus derived from nasopharyngeal carcinoma and its similarity to other Epstein-Barr virus isolates.
    Gene. 1984 Mar;27(3):279-88 PMID: 6329907
  28. DNA sequence and expression of the B95-8 Epstein-Barr virus genome.
    Nature. 1984 Jul 19-25;310(5974):207-11 PMID: 6087149
  29. Mapping of Epstein-Barr virus proteins on the genome by translation of hybrid-selected RNA from induced P3HR1 cells and induced Raji cells.
    Virology. 1985 Feb;141(1):1-13 PMID: 2983491
  30. A putative transforming gene of Jijoye virus differs from that of Epstein-Barr virus prototypes.
    Virology. 1985 Mar;141(2):221-34 PMID: 3002016
  31. Latent and lytic cycle promoters of Epstein-Barr virus.
    EMBO J. 1983;2(8):1331-8 PMID: 10872327
  32. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
  33. Origin of periodic proteins.
    Fed Proc. 1976 Aug;35(10):2139-40 PMID: 947792
  34. Sequence data handling by computer.
    Nucleic Acids Res. 1977 Nov;4(11):4037-51 PMID: 593900
  35. Alleles of the fibroin gene coding for proteins of different lengths.
    Cell. 1979 Jun;17(2):407-13 PMID: 455471
  36. Biological and biochemical observations on isolates of EB virus from the malignant epithelial cells of two nasopharyngeal carcinomas.
    Int J Cancer. 1979 Sep 15;24(3):294-302 PMID: 226484
  37. Genetic polymorphism of silk fibroin studied by two-dimensional translation pause fingerprints.
    Cell. 1979 Oct;18(2):581-9 PMID: 498285
  38. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  39. Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping.
    Methods Enzymol. 1980;65(1):718-49 PMID: 6154876
  40. Transposable elements.
    Cell. 1980 Jul;20(3):579-95 PMID: 6251969
  41. The structure and evolution of the human beta-globin gene family.
    Cell. 1980 Oct;21(3):653-68 PMID: 6985477
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.56.3.987-995.1985
Published
1985-12-00
Pages
987-95
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC252673
Subset
IM
Databases
GENBANK
K03330, K03331, M11658
External Links
PubMed source DOI: 10.1128/JVI.56.3.987-995.1985 PMC Full Text View journal details
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