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PMID: 3000772 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The release of growth arrest by microinjection of adenovirus E1A DNA.

The EMBO journal ·Vol. 4 ·No. 9 ·1985-09-00 ·Pages 2329-36

Stabel S, Argos P, Philipson L

Abstract

The induction of DNA synthesis in growth-arrested mouse fibroblasts (NIH 3T3) was studied by microinjection of different constructs of adenovirus DNA using SV40 DNA and plasmid DNA as positive and negative controls. The E1A region of adenovirus types 2 and 12 appears to be sufficient to induce cellular DNA synthesis after growth arrest in approximately 30% of the cells and both 13S and 12S cDNA constructs mediate this effect. The presence of the E1A protein products as assayed by immunofluorescence does not strictly correlate with the induction of DNA synthesis in microinjected cells in contrast to the SV40 large T-antigen. Microinjection of truncated fragments of the Ad12 E1A region suggests, however, that the protein products of 12S and 13S may be involved in the induction process. A sequence comparison of the SV40 T-antigen and the adenovirus E1A products identified a region of significant homology providing a basis for a hypothesis concerning the evolution of T-antigen genes in DNA viruses.

MeSH Terms
Adenoviruses, Human/genetics Animals Antigens, Polyomavirus Transforming Antigens, Viral, Tumor/genetics Cell Division Cells, Cultured DNA Replication DNA, Viral/genetics Fibroblasts/cytology Mice Mice, Inbred Strains Microinjections Oncogene Proteins, Viral/genetics Plasmids Simian virus 40/genetics
Chemicals
Antigens, Polyomavirus Transforming Antigens, Viral, Tumor DNA, Viral Oncogene Proteins, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Stabel S
Argos P
Philipson L
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-09-00
Pages
2329-36
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554505
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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