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PMID: 3001724 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular analysis of a deletion mutant provirus of type I human T-cell lymphotropic virus: evidence for a doubly spliced x-lor mRNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 1 ·1986-01-00 ·Pages 38-42

Aldovini A, De Rossi A, Feinberg MB, Wong-Staal F, Franchini G

Abstract

The genome of the human T-cell leukemia/lymphotropic virus type I (HTLV-I) contains a functional gene denominated x-lor that may be important in HTLV-I transformation of human T cells. To study the role of x-lor and other HTLV-I genes in cellular transformation, we obtained a transformed nonproducer human T-cell line containing a single defective HTLV-I provirus (HTLV-I 55/PL). This 7-kilobase provirus had undergone a deletion involving the entire envelope gene and the nonconserved region. The point of the deletion corresponded to the junction of a donor splice site, located between the polymerase gene and the envelope gene (nucleotide 5183), and the acceptor site for the mRNA of the x-lor gene (nucleotide 7302). The juxtaposition of nucleotides 5182 and 7302 brings the initiating methionine codon of the envelope gene immediately 5' to the x-lor region, leaving the DNA sequence in frame for expression of a protein product. This finding suggests that a double splicing mechanism is used to express the x-lor gene, and that the defective provirus 55/PL was generated through the reverse transcription of a partially spliced mRNA. Analysis of the x-lor mRNA of other HTLV-I-transformed cell lines revealed that a double splicing process is commonly used. Furthermore, since 55/PL can be faithfully transmitted and is able to immortalize recipient T cells, we can conclude that the envelope gene is not necessary for in vitro transformation by HTLV-I.

MeSH Terms
Base Sequence Cell Line Cell Transformation, Viral DNA, Recombinant DNA, Viral/genetics Deltaretrovirus/genetics Genes, Viral Immunosorbent Techniques Mutation Nucleic Acid Hybridization RNA, Messenger/genetics RNA, Viral/genetics T-Lymphocytes/microbiology Viral Envelope Proteins/genetics
Chemicals
DNA, Recombinant DNA, Viral RNA, Messenger RNA, Viral Viral Envelope Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Aldovini A
De Rossi A
Feinberg M B
Wong-Staal F
Franchini G
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43 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.1.38
Published
1986-01-00
Pages
38-42
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC322786
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.83.1.38 PMC Full Text View journal details
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