Molecular cloning of genetic determinants for inhibition of fungal growth by a fluorescent pseudomonad.

Journal of bacteriology ·Vol. 165 ·No. 3 ·1986-03-00 ·Pages 696-703

Gutterson NI, Layton TJ, Ziegle JS, Warren GJ

Abstract

Pseudomonas fluorescens HV37a inhibits growth of the fungus Pythium ultimum in vitro. Optimal inhibition is observed on potato dextrose agar, a rich medium. Mutations eliminating fungal inhibition were obtained after mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine. Mutants were classified by cosynthesis and three groups were distinguished, indicating that a minimum of three genes are required for fungal inhibition. Cosmids that contain wild-type alleles of the genes were identified in an HV37a genomic library by complementation of the respective mutants. This analysis indicated that three distinct genomic regions were required for fungal inhibition. The cosmids containing these loci were mapped by transposon insertion mutagenesis. Two of the cosmids were found to contain at least two genes each. Therefore, at least five genes in HV37a function as determinants of fungal inhibition.

MeSH Terms

Alleles Antibiosis Antifungal Agents/metabolism Chytridiomycota/growth & development Cloning, Molecular Cosmids DNA Transposable Elements Genes, Bacterial Genetic Complementation Test Mutation Pseudomonas fluorescens/genetics,metabolism Pythium/growth & development

Chemicals

Antifungal Agents DNA Transposable Elements

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Gutterson N I

Layton T J

Ziegle J S

Warren G J

References (12)

12 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.165.3.696-703.1986

Published

1986-03-00

Pages

696-703

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC214485

Subset

External Links

PubMed source DOI: 10.1128/jb.165.3.696-703.1986 PMC Full Text View journal details

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Molecular cloning of genetic determinants for inhibition of fungal growth by a fluorescent pseudomonad.

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