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PMID: 3005977 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A human DNA-binding protein is methylation-specific and sequence-specific.

Nucleic acids research ·Vol. 14 ·No. 4 ·1986-02-25 ·Pages 1599-614

Wang RY, Zhang XY, Ehrlich M

Abstract

A nuclear protein isolated from human placenta, methylated DNA-binding protein (MDBP), binds selectively to DNA enriched in 5-methylcytosine. We now demonstrate that MDBP is a sequence-specific, as well as methylation-specific, DNA-binding protein. From ten restriction fragments of pBR322 DNA methylated with human DNA methyltransferase, one was bound to MDBP very much more strongly than any of the others. For this preferential binding to MDBP, the DNA had to be methylated. By a DNase I protection experiment (DNase I footprinting), a 22-base sequence within this methylated restriction fragment was shown to be specifically protected by MDBP. The sequence-specificity of MDBP coupled with its dependence on DNA methylation suggests that this is one of the proteins which modulates important functions of human DNA methylation in vivo.

MeSH Terms
5-Methylcytosine Base Sequence Binding Sites Cloning, Molecular Cytosine/analogs & derivatives,metabolism DNA-Binding Proteins/metabolism Endonucleases Gene Expression Regulation Humans Methylation Placenta/analysis Single-Strand Specific DNA and RNA Endonucleases
Chemicals
DNA-Binding Proteins 5-Methylcytosine Cytosine Endonucleases Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wang R Y
Zhang X Y
Ehrlich M
References (36)
36 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.4.1599
Published
1986-02-25
Pages
1599-614
Language
English
Region
England
NLM ID
0411011
PMCID
PMC339531
Subset
IM
Grants
NCI NIH HHS · CA-19942 · United States
External Links
PubMed source DOI: 10.1093/nar/14.4.1599 PMC Full Text View journal details
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