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PMID: 3010232 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Supercoil induced S1 hypersensitive sites in the rat and human ribosomal RNA genes.

Nucleic acids research ·Vol. 14 ·No. 8 ·1986-04-25 ·Pages 3263-77

Financsek I, Tora L, Kelemen G, Hidvégi EJ

Abstract

Rat and human ribosomal RNA gene fragments in supercoiled plasmids were examined for S1 nuclease hypersensitivity. In the transcribed portion of genes the number and distribution of S1 sites were found to be species specific. No S1 sites were detected in the promoter regions. In the nontranscribed spacer (NTS), downstream of the 3' end of 28S RNA gene, S1 sites appear to be conserved in rat and human rDNAs. A rat NTS fragment (2987 nucleotides long), containing three S1 sites was sequenced and the S1 sites in this region were localized in polypyrimidine . polypurine simple repeat sequences. Other types of simple sequences, two type 2 Alu repeats and an ID sequence were also found in the sequenced region. The possible role of simple sequences and S1 sites in transcription and in recombination events of rDNA is discussed.

MeSH Terms
Animals Base Sequence Cloning, Molecular DNA, Ribosomal/genetics DNA, Superhelical/genetics Endonucleases/metabolism Genes Humans Nucleic Acid Hybridization Plasmids Protein Biosynthesis RNA, Ribosomal/genetics Rats Single-Strand Specific DNA and RNA Endonucleases Species Specificity
Chemicals
DNA, Ribosomal DNA, Superhelical RNA, Ribosomal Endonucleases Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Financsek I
Tora L
Kelemen G
Hidvégi E J
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43 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.8.3263
Published
1986-04-25
Pages
3263-77
Language
English
Region
England
NLM ID
0411011
PMCID
PMC339761
Subset
IM
Databases
GENBANK
X03838
External Links
PubMed source DOI: 10.1093/nar/14.8.3263 PMC Full Text View journal details
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