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PMID: 3011738 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of the surface exclusion genes traS and traT from the IncF0 lac plasmid pED208.

Journal of bacteriology ·Vol. 166 ·No. 3 ·1986-06-00 ·Pages 713-21

Finlay BB, Paranchych W

Abstract

pED208 is a 90-kilobase conjugative plasmid belonging to the incompatibility group IncF0 lac. The surface exclusion system from this plasmid was cloned and sequenced, and two genes demonstrated exclusion ability. traS encoded a 186-amino-acid hydrophobic protein which, when transcribed from a vector promoter, caused exclusion of pED208. The product of traT (TraTp) was a 245-residue protein which was highly expressed independently of a vector promoter in Escherichia coli minicells. The TraTp from pED208 was homologous with traT products from the IncF plasmids R-100 and F (80% homology), but recombinants containing the pED208 surface exclusion system excluded F poorly.

MeSH Terms
Base Sequence Cloning, Molecular Cross Reactions DNA Restriction Enzymes/metabolism DNA, Bacterial/analysis Deoxyribonuclease HindIII Deoxyribonucleases, Type II Site-Specific Genes, Bacterial Molecular Weight Plasmids Protein Conformation
Chemicals
DNA, Bacterial DNA Restriction Enzymes Deoxyribonuclease HindIII CTCGAG-specific type II deoxyribonucleases Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Finlay B B
Paranchych W
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.166.3.713-721.1986
Published
1986-06-00
Pages
713-21
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215180
Subset
IM
Databases
GENBANK
M13465
External Links
PubMed source DOI: 10.1128/jb.166.3.713-721.1986 PMC Full Text View journal details
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