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PMID: 3011750 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Mutations in the araC regulatory gene of Escherichia coli B/r that affect repressor and activator functions of AraC protein.

Journal of bacteriology ·Vol. 166 ·No. 3 ·1986-06-00 ·Pages 892-900

Cass LG, Wilcox G

Abstract

Mutations in the araC gene of Escherichia coli B/r were isolated which alter both activation of the araBAD operon expression and autoregulation. The mutations were isolated on an araC-containing plasmid by hydroxylamine mutagenesis of plasmid DNA. The mutant phenotype selected was the inability to autoregulate. The DNA sequence of 16 mutants was determined and found to consist of seven different missense mutations located within the distal third of the araC gene. Enzyme activities revealed that each araC mutation had altered both autoregulatory and activator functions of AraC protein. The mutational analysis presented in this paper suggests that both autoregulatory and activator functions are localized to the same determinants of the AraC protein and that the amino acid sequence within the carboxy-terminal region of AraC protein is important for site-specific DNA binding.

MeSH Terms
Arabinose/metabolism Autoradiography Bacterial Proteins/metabolism Base Sequence DNA, Bacterial/metabolism Escherichia coli/genetics Genes, Regulator Immunosorbent Techniques Mutation Operon Structure-Activity Relationship Transcription, Genetic Trypsin/metabolism
Chemicals
Bacterial Proteins DNA, Bacterial Arabinose Trypsin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cass L G
Wilcox G
References (41)
41 references, click to expand
  1. An analysis of "revertants" of a deletion mutant in the C gene of the L-arabinose gene complex in Escherichia coli B-r: isolation of initiator constitutive mutants (Ic).
    J Mol Biol. 1969 Jul 28;43(2):281-98 PMID: 4896373
  2. Purification of the araC protein.
    Proc Natl Acad Sci U S A. 1971 Sep;68(9):2145-8 PMID: 4943786
  3. Initiator constitutive mutants of the L-arabinose operon (OIBAD) of Escherichia coli B/r.
    Genetics. 1971 Nov;69(3):289-302 PMID: 4944585
  4. Direction of transcription of the regulatory gene araC in Escherichia coli B-r.
    Proc Natl Acad Sci U S A. 1974 Sep;71(9):3635-9 PMID: 4610582
  5. Regulation: positive control.
    Annu Rev Genet. 1974;8:219-42 PMID: 4374117
  6. Uniform nomenclature for bacterial plasmids: a proposal.
    Bacteriol Rev. 1976 Mar;40(1):168-89 PMID: 1267736
  7. Stabilization and size of araC protein.
    Mol Gen Genet. 1976 Apr 23;145(1):97-100 PMID: 775315
  8. Regulation of the regulatory gene for the arabinose pathway, araC.
    J Mol Biol. 1976 Jul 5;104(3):557-66 PMID: 781294
  9. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  10. Escherichia coli K-12 mutants deficient in uracil-DNA glycosylase.
    J Bacteriol. 1978 Jun;134(3):1039-45 PMID: 350837
  11. Nucleotide sequence of the L-arabinose regulatory region of Escherichia coli K12.
    J Biol Chem. 1978 Oct 10;253(19):6931-3 PMID: 357433
  12. pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long.
    Nucleic Acids Res. 1978 Aug;5(8):2721-8 PMID: 358147
  13. DNA sequence of the araBAD promoter in Escherichia coli B/r.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):4724-8 PMID: 368797
  14. A dimer of AraC protein contacts three adjacent major groove regions of the araI DNA site.
    Proc Natl Acad Sci U S A. 1985 May;82(10):3129-33 PMID: 3858809
  15. Altered DNA contacts made by a mutant AraC protein.
    Nucleic Acids Res. 1985 Jul 25;13(14):5019-26 PMID: 3895160
  16. Dominant negative mutations in the Tn10 tet repressor: evidence for use of the conserved helix-turn-helix motif in DNA binding.
    Proc Natl Acad Sci U S A. 1985 Sep;82(18):6226-30 PMID: 2994067
  17. araB Gene and nucleotide sequence of the araC gene of Erwinia carotovora.
    J Bacteriol. 1985 Nov;164(2):717-22 PMID: 3902795
  18. Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4530-3 PMID: 159458
  19. The Escherichia coli L-arabinose operon: binding sites of the regulatory proteins and a mechanism of positive and negative regulation.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3346-50 PMID: 6251457
  20. The araC regulatory gene mRNA contains a leader sequence.
    Mol Gen Genet. 1980;180(1):219-26 PMID: 6160371
  21. DNA sequence of the araC regulatory gene from Escherichia coli B/r.
    Nucleic Acids Res. 1980 Nov 25;8(22):5267-74 PMID: 7008027
  22. In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals.
    J Bacteriol. 1980 Aug;143(2):971-80 PMID: 6162838
  23. Mechanism of araC autoregulation and the domains of two overlapping promoters, Pc and PBAD, in the L-arabinose regulatory region of Escherichia coli.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):752-6 PMID: 6262769
  24. Construction and characterization of new cloning vehicles. V. Mobilization and coding properties of pBR322 and several deletion derivatives including pBR327 and pBR328.
    Gene. 1981 Jan-Feb;13(1):25-35 PMID: 6263753
  25. The araC gene of Escherichia coli: transcriptional and translational start-points and complete nucleotide sequence.
    Gene. 1980 Dec;12(3-4):179-90 PMID: 7019009
  26. DNA sequence of the araBAD-araC controlling region in Salmonella typhimurium LT2.
    Gene. 1981 Sep;14(4):309-19 PMID: 7028569
  27. Solid phase synthesis of polynucleotides. VI. Further studies on polystyrene copolymers for the solid support.
    Nucleic Acids Res. 1982 Mar 11;10(5):1755-69 PMID: 7071021
  28. A set of synthetic oligodeoxyribonucleotide primers for DNA sequencing in the plasmid vector pBR322.
    Gene. 1981 Dec;16(1-3):21-6 PMID: 6282692
  29. Structural similarity in the DNA-binding domains of catabolite gene activator and cro repressor proteins.
    Proc Natl Acad Sci U S A. 1982 May;79(10):3097-100 PMID: 6212926
  30. Structure of catabolite gene activator protein at 2.9-A resolution. Incorporation of amino acid sequence and interactions with cyclic AMP.
    J Biol Chem. 1982 Aug 25;257(16):9518-24 PMID: 6286624
  31. The nucleotide sequence of the araC regulatory gene in Salmonella typhimurium LT2.
    Gene. 1982 May;18(2):157-63 PMID: 6751938
  32. In vivo regulation of the Escherichia coli araC promoter.
    J Bacteriol. 1983 Aug;155(2):593-600 PMID: 6307972
  33. Linkage map of Escherichia coli K-12, edition 7.
    Microbiol Rev. 1983 Jun;47(2):180-230 PMID: 6348505
  34. Molecular cloning and expression of gene fragments from corynebacteriophage beta encoding enzymatically active peptides of diphtheria toxin.
    J Bacteriol. 1983 Nov;156(2):680-5 PMID: 6313613
  35. Functional limits of the araIc promoter suggest an additional regulatory site for araBAD expression.
    J Bacteriol. 1984 Apr;158(1):141-7 PMID: 6370954
  36. Model of specific complex between catabolite gene activator protein and B-DNA suggested by electrostatic complementarity.
    Proc Natl Acad Sci U S A. 1984 Jul;81(13):3973-7 PMID: 6377305
  37. Regulation of the araC gene of Escherichia coli: catabolite repression, autoregulation, and effect on araBAD expression.
    Proc Natl Acad Sci U S A. 1984 Jul;81(13):4120-4 PMID: 6377308
  38. An operator at -280 base pairs that is required for repression of araBAD operon promoter: addition of DNA helical turns between the operator and promoter cyclically hinders repression.
    Proc Natl Acad Sci U S A. 1984 Aug;81(16):5017-20 PMID: 6089170
  39. Protein-DNA recognition.
    Annu Rev Biochem. 1984;53:293-321 PMID: 6236744
  40. Regulation of the Escherichia coli L-arabinose operon studied by gel electrophoresis DNA binding assay.
    J Mol Biol. 1984 Sep 25;178(3):611-28 PMID: 6387154
  41. Mutational studies with the trp repressor of Escherichia coli support the helix-turn-helix model of repressor recognition of operator DNA.
    Proc Natl Acad Sci U S A. 1985 Jan;82(2):483-7 PMID: 3881764
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.166.3.892-900.1986
Published
1986-06-00
Pages
892-900
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215210
Subset
IM
Grants
NIGMS NIH HHS · GM30896 · United States
External Links
PubMed source DOI: 10.1128/jb.166.3.892-900.1986 PMC Full Text View journal details
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