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PMID: 3014501 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Replication of a plasmid bearing a human Alu-family repeat in monkey COS-7 cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 13 ·1986-07-00 ·Pages 4660-4

Johnson EM, Jelinek WR

Abstract

Monkey COS-7 cells were transformed with BLUR8 DNA, a pBR322 plasmid containing a human Alu-family sequence at the BamHI site. Within 24 hr of transformation 2-5% of the BLUR8 molecules recovered resisted cleavage by Dpn I, indicating they had replicated. Electron microscopy revealed appropriately sized circular molecules with replication bubbles whose centers were mapped to the Alu insert. A 16-base-pair deletion within the Alu sequence prevented replication. The results indicate that certain Alu sequences can serve as origins of replication in COS-7 cells.

MeSH Terms
Animals Antigens, Viral, Tumor/genetics Base Sequence Cell Line Chlorocebus aethiops Chromosome Mapping DNA Replication Humans Plasmids Repetitive Sequences, Nucleic Acid Simian virus 40/genetics Transfection
Chemicals
Antigens, Viral, Tumor
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Johnson E M
Jelinek W R
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30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.13.4660
Published
1986-07-00
Pages
4660-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323801
Subset
IM
Grants
NIGMS NIH HHS · GM26170 · United States
NIGMS NIH HHS · GM30363 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.13.4660 PMC Full Text View journal details
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