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PMID: 3018487 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Sequence-dependent DNA replication in preimplantation mouse embryos.

Molecular and cellular biology ·Vol. 5 ·No. 11 ·1985-11-00 ·Pages 2924-35

Wirak DO, Chalifour LE, Wassarman PM, Muller WJ, Hassell JA, DePamphilis ML

Abstract

Circular, double-stranded DNA molecules were injected into nuclei of mouse oocytes and one- or two-cell embryos to determine whether specific sequences were required to replicate DNA during mouse development. Although all of the injected DNAs were stable, replication of plasmid pML-1 DNA was not detected unless it contained either polyomavirus (PyV) or simian virus 40 (SV40) DNA sequences. Replication occurred in embryos, but not in oocytes. PyV DNA, either alone or recombined with pML-1, underwent multiple rounds of replication to produce superhelical and relaxed circular monomers after injection into one- or two-cell embryos. SV40 DNA also replicated, but only 3% as well as PyV DNA. Coinjection of PyV DNA with either pML-1 or SV40 had no effect on the replicating properties of the three DNAs. These results are consistent with a requirement for specific cis-acting sequences to replicate DNA in mammalian embryos, in contrast to sequence-independent replication of DNA injected into Xenopus eggs. Furthermore, PyV DNA replication in mouse embryos required PyV large T-antigen and either the alpha-beta-core or beta-core configuration of the PyV origin of replication. Although the alpha-core configuration replicated in differentiated mouse cells, it failed to replicate in mouse embryos, demonstrating cell-specific activation of an origin of replication. Replication or expression of PyV DNA interfered with normal embryonic development. These results reveal that mouse embryos are permissive for PyV DNA replication, in contrast to the absence of PyV DNA replication and gene expression in mouse embryonal carcinoma cells.

MeSH Terms
Animals Blastocyst/cytology,metabolism Cell Line Cells, Cultured Chlorocebus aethiops DNA Replication DNA, Viral/genetics Kidney Mice Microinjections Nucleic Acid Hybridization Oocytes/cytology Plasmids Simian virus 40/genetics
Chemicals
DNA, Viral
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Wirak D O
Chalifour L E
Wassarman P M
Muller W J
Hassell J A
DePamphilis M L
References (79)
79 references, click to expand
  1. Heat shock proteins, first major products of zygotic gene activity in mouse embryo.
    Nature. 1983 Sep 22-28;305(5932):331-3 PMID: 6684733
  2. The new yeast genetics.
    Nature. 1983 Sep 29-Oct 5;305(5933):391-7 PMID: 6353245
  3. DNA fragments from F9 PyEC mutants increase expression of heterologous genes in transfected F9 cells.
    Cell. 1983 Dec;35(3 Pt 2):693-9 PMID: 6317199
  4. Gene amplification in a single cell cycle in Chinese hamster ovary cells.
    J Biol Chem. 1984 Feb 10;259(3):1901-10 PMID: 6693439
  5. Changes in gene position are accompanied by a change in time of replication.
    Cell. 1984 Mar;36(3):689-96 PMID: 6421490
  6. DNA binding activity of polyoma virus large tumor antigen.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):1941-5 PMID: 6326093
  7. Regulated replication of DNA microinjected into eggs of Xenopus laevis.
    Cell. 1980 Oct;21(3):761-71 PMID: 6254667
  8. Mutation near the polyoma DNA replication origin permits productive infection of F9 embryonal carcinoma cells.
    Cell. 1981 Mar;23(3):809-14 PMID: 6261957
  9. Isolation and characterization of polyoma host range mutants that replicate in nullipotential embryonal carcinoma cells.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):1100-4 PMID: 6262755
  10. Inhibition of SV40 replication in simian cells by specific pBR322 DNA sequences.
    Nature. 1981 Sep 3;293(5827):79-81 PMID: 6267479
  11. Polyoma virus infection of retinoic acid-induced differentiated teratocarcinoma cells.
    J Virol. 1981 Jul;39(1):306-12 PMID: 6268818
  12. Fine structure of the origin-proximal DNAase I-hypersensitive region in wild-type and EC mutant polyoma.
    Cell. 1981 Sep;25(3):651-8 PMID: 6269751
  13. The molecular and cellular basis of preimplantation mouse development.
    Biol Rev Camb Philos Soc. 1981 Aug;56(3):463-98 PMID: 7030421
  14. Fate of viral DNA in nonpermissive cells infected with simian virus 40.
    Proc Natl Acad Sci U S A. 1981 Nov;78(11):6638-42 PMID: 6273879
  15. A region of the polyoma virus genome between the replication origin and late protein coding sequences is required in cis for both early gene expression and viral DNA replication.
    Nucleic Acids Res. 1981 Dec 11;9(23):6231-50 PMID: 6275353
  16. A small segment of polyoma virus DNA enhances the expression of a cloned beta-globin gene over a distance of 1400 base pairs.
    Nucleic Acids Res. 1981 Dec 11;9(23):6251-64 PMID: 6275354
  17. Sequences at the capped 5'-ends of polyoma virus late region mRNAs: an example of extreme terminal heterogeneity.
    Nucleic Acids Res. 1981 Dec 11;9(23):6305-22 PMID: 6275357
  18. Regulation of metallothionein--thymidine kinase fusion plasmids injected into mouse eggs.
    Nature. 1982 Mar 4;296(5852):39-42 PMID: 6278311
  19. Polyoma mutants that productively infect F9 embryonal carcinoma cells do not rescue wild-type polyoma in F9 cells.
    Proc Natl Acad Sci U S A. 1982 Mar;79(5):1479-83 PMID: 6280185
  20. Tumor viruses and early mouse embryos.
    Biochim Biophys Acta. 1982 Apr 29;651(2-3):105-41 PMID: 6284213
  21. Cytoplasmic control of preimplantation development in vitro in the mouse.
    Nature. 1982 Sep 30;299(5882):460-2 PMID: 6896904
  22. Sequence specificity for the initiation of RNA-primed simian virus 40 DNA synthesis in vivo.
    J Mol Biol. 1984 May 15;175(2):131-57 PMID: 6202875
  23. Detection of DNA and RNA virus genomes in organ systems of whole mice: patterns of mouse organ infection by polyomavirus.
    J Virol. 1984 Jun;50(3):779-83 PMID: 6328007
  24. Initiation of SV40 DNA replication after microinjection into Xenopus eggs.
    Biochim Biophys Acta. 1984 Jul 18;782(3):295-303 PMID: 6329301
  25. The transition from maternal to embryonic control in the 2-cell mouse embryo.
    EMBO J. 1982;1(6):681-6 PMID: 7188357
  26. Lack of specific sequence requirement for DNA replication in Xenopus eggs compared with high sequence specificity in yeast.
    Cell. 1984 Aug;38(1):55-64 PMID: 6380762
  27. The units of DNA replication in Drosophila melanogaster chromosomes.
    Cold Spring Harb Symp Quant Biol. 1974;38:205-23 PMID: 4208784
  28. Meiotic maturation of the mammalian oocyte in vitro: effect of dibutyryl cyclic AMP on protein synthesis.
    J Exp Zool. 1974 Aug;189(2):275-81 PMID: 4368902
  29. Neoplastic differentiation: interaction of simian virus 40 and polyoma virus with murine teratocarcinoma cells in vitro.
    J Cell Physiol. 1975 Apr;85(2 Pt 1):179-87 PMID: 164473
  30. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  31. "Early" simian-virus-40-specific RNA contains information for tumor antigen formation and chromatin replication.
    Proc Natl Acad Sci U S A. 1976 Feb;73(2):366-70 PMID: 174105
  32. Electron microscopic analysis of DNA replication in main band and satellite DNAs of Drosophila virilis.
    J Mol Biol. 1976 Dec;108(2):305-31 PMID: 1011257
  33. Assembly of SV40 chromatin in a cell-free system from Xenopus eggs.
    Cell. 1977 Feb;10(2):237-43 PMID: 189936
  34. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  35. Complementary specificity of restriction endonucleases of Diplococcus pneumoniae with respect to DNA methylation.
    J Mol Biol. 1977 Jul;114(1):153-68 PMID: 20509
  36. RNA primers in SV40 DNA replication: identification of transient RNA-DNA covalent linkages in replicating DNA.
    Biochemistry. 1977 Nov 15;16(23):4990-8 PMID: 199246
  37. Electron microscopic analysis of chromatin replication in the cellular blastoderm Drosophila melanogaster embryo.
    Cell. 1977 Nov;12(3):795-804 PMID: 411576
  38. The beneficial effect EDTA on development of mouse one-cell embryos in chemically defined medium.
    Dev Biol. 1977 Dec;61(2):378-83 PMID: 412719
  39. DNA synthesis in the second and third cell cycles of mouse preimplantation development. A cytophotometric study.
    Exp Cell Res. 1978 Mar 1;112(1):199-205 PMID: 631211
  40. Oocyte development in the mouse: an ultrastructural comparison of oocytes isolated at various stages of growth and meiotic competence.
    J Morphol. 1978 May;156(2):209-35 PMID: 642015
  41. Discontinuous DNA replication: accumulation of Simian virus 40 DNA at specific stages in its replication.
    J Mol Biol. 1978 Apr 15;120(3):401-22 PMID: 206700
  42. Selective DNA conservation and chromatin assembly after injection of SV40 DNA into Xenopus oocytes.
    Dev Biol. 1978 May;64(1):178-88 PMID: 207607
  43. [Susceptibility of teratocarcinoma cells to simian virus 40 and polyoma virus (author's transl)].
    Ann Microbiol (Paris). 1978 Feb-Mar;129(2):227-38 PMID: 209713
  44. Evidence for non-spliced SV40 RNA in undifferentiated murine teratocarcinoma stem cells.
    Nature. 1979 Jul 26;280(5720):335-8 PMID: 223058
  45. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
  46. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  47. The use of intensifying screens or organic scintillators for visualizing radioactive molecules resolved by gel electrophoresis.
    Methods Enzymol. 1980;65(1):363-71 PMID: 7374459
  48. Teratocarcinomas and mammalian embryogenesis.
    Science. 1980 Aug 15;209(4458):768-76 PMID: 6250214
  49. Assembly of transcriptionally active chromatin in Xenopus oocytes requires specific DNA binding factors.
    Cell. 1984 Sep;38(2):511-21 PMID: 6540626
  50. Gene amplification.
    Annu Rev Biochem. 1984;53:447-91 PMID: 6383198
  51. Multiple binding sites for polyomavirus large T antigen within regulatory sequences of polyomavirus DNA.
    J Virol. 1984 Dec;52(3):750-60 PMID: 6092707
  52. Polyoma virus DNA replication requires an enhancer.
    Nature. 1984 Nov 15-21;312(5991):242-6 PMID: 6095089
  53. Two distinct enhancers with different cell specificities coexist in the regulatory region of polyoma.
    Cell. 1984 Dec;39(3 Pt 2):653-62 PMID: 6096017
  54. Location of sequences in polyomavirus DNA that are required for early gene expression in vivo and in vitro.
    Mol Cell Biol. 1984 Dec;4(12):2594-609 PMID: 6098813
  55. Cloned mouse DNA fragments can replicate in a simian virus 40 T antigen-dependent system in vivo and in vitro.
    Mol Cell Biol. 1985 Mar;5(3):563-8 PMID: 2985958
  56. Host species specificity of polyomavirus DNA replication is not altered by simian virus 40 72-base-pair repeats.
    Mol Cell Biol. 1985 Jun;5(6):1534-7 PMID: 2993870
  57. DNA sequences required for specific and efficient initiation of transcription at the polyoma virus early promoter.
    Mol Cell Biol. 1982 Jul;2(7):737-51 PMID: 6100910
  58. Template structural requirements for transcription in vivo by RNA polymerase II.
    Mol Cell Biol. 1982 Dec;2(12):1595-607 PMID: 14582200
  59. Lactate dehydrogenase activity in the preimplanted mouse embryo.
    Biochim Biophys Acta. 1965 Nov 22;110(2):439-41 PMID: 4286293
  60. Selective extraction of polyoma DNA from infected mouse cell cultures.
    J Mol Biol. 1967 Jun 14;26(2):365-9 PMID: 4291934
  61. Enchancement of the infectivity of simian virus 40 deoxyribonucleic acid with diethylaminoethyl-dextran.
    J Natl Cancer Inst. 1968 Aug;41(2):351-7 PMID: 4299537
  62. Complete development in vitro of the pre-implantation stages of the mouse in a simple chemically defined medium.
    J Reprod Fertil. 1968 Nov;17(2):399-401 PMID: 5749384
  63. Pronuclear DNA synthesis in mouse eggs. An autoradiographic study.
    Exp Cell Res. 1973 Nov;82(1):143-51 PMID: 4751978
  64. Dissection of a replication origin of Xenopus DNA.
    Proc Natl Acad Sci U S A. 1982 Sep;79(18):5572-6 PMID: 6752953
  65. A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
    Cell. 1982 Oct;30(3):675-86 PMID: 6183003
  66. A major developmental transition in early Xenopus embryos: II. Control of the onset of transcription.
    Cell. 1982 Oct;30(3):687-96 PMID: 7139712
  67. Physical and biological features of polyoma virus mutants able to infect embryonal carcinoma cell lines.
    J Virol. 1982 Sep;43(3):800-8 PMID: 6292462
  68. Non-contiguous segments of the polyoma genome required in cis for DNA replication.
    J Mol Biol. 1982 Nov 15;161(4):533-50 PMID: 6296399
  69. Poly(A) length, cytoplasmic adenylation and synthesis of poly(A)+ RNA in early mouse embryos.
    Dev Biol. 1983 Feb;95(2):331-41 PMID: 6186546
  70. Transcriptional 'enhancers' from SV40 and polyoma virus show a cell type preference.
    Nucleic Acids Res. 1982 Dec 20;10(24):7965-76 PMID: 6298703
  71. ARS replication during the yeast S phase.
    Cell. 1983 Mar;32(3):831-8 PMID: 6339074
  72. Cloning of the initiation region of a mammalian chromosomal replicon.
    Nature. 1983 Mar 31-Apr 6;302(5907):439-41 PMID: 6300683
  73. Novel amplification and transcriptional activity of chorion genes in Drosophila melanogaster follicle cells.
    Cell. 1983 Jun;33(2):543-53 PMID: 6407758
  74. DNA replication origin of polyoma virus: early proximal boundary.
    J Virol. 1983 Jul;47(1):244-8 PMID: 6306277
  75. Regulation of polyoma virus transcription in murine embryonal carcinoma cells.
    J Virol. 1983 Jul;47(1):55-64 PMID: 6306281
  76. Spontaneous formation of nucleus-like structures around bacteriophage DNA microinjected into Xenopus eggs.
    Cell. 1983 Aug;34(1):13-23 PMID: 6224569
  77. Polyomavirus origin for DNA replication comprises multiple genetic elements.
    J Virol. 1983 Sep;47(3):586-99 PMID: 6312083
  78. Polyomavirus large T antigen binds independently to multiple, unique regions on the viral genome.
    J Virol. 1983 Sep;47(3):600-10 PMID: 6312084
  79. Comparison of the DNA sequence of the Crawford small-plaque variant of polyomavirus with those of polyomaviruses A2 and strain 3.
    J Virol. 1983 Nov;48(2):472-80 PMID: 6312103
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.11.2924-2935.1985
Published
1985-11-00
Pages
2924-35
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369103
Subset
IM
Grants
NICHD NIH HHS · HD-12275 · United States
External Links
PubMed source DOI: 10.1128/mcb.5.11.2924-2935.1985 PMC Full Text View journal details
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