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PMID: 6570189 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Sequence-specific binding of simian virus 40 A protein to nonorigin and cellular DNA.

Molecular and cellular biology ·Vol. 4 ·No. 12 ·1984-12-00 ·Pages 2631-8

Wright PJ, DeLucia AL, Tegtmeyer P

Abstract

The simian virus 40 A protein (T antigen) recognized and bound to the consensus sequence 5'-GAGGC-3' in DNA from many sources. Sequence-specific binding to single pentanucleotides in randomly chosen DNA predominated over binding to nonspecific sequences. The asymmetric orientation of protein bound to nonorigin recognition sequences also resembled that of protein bound to the origin region of simian virus 40 DNA. Sequence variations in the DNA adjacent to single pentanucleotides influenced binding affinities even though methylation interference and protection studies did not reveal specific interactions outside of pentanucleotides. Thus, potential locations of A protein bound to any DNA can be predicted although the determinants of binding affinity are not yet understood. Sequence-specific binding of A protein to cellular DNA would provide a mechanism for specific alterations of host gene expression that facilitate viral function.

MeSH Terms
Antigens, Polyomavirus Transforming Antigens, Viral, Tumor/metabolism Base Sequence Binding Sites DNA/metabolism DNA, Viral/metabolism Deoxyribonucleases/metabolism Humans Viral Proteins/metabolism
Chemicals
Antigens, Polyomavirus Transforming Antigens, Viral, Tumor DNA, Viral Viral Proteins DNA Deoxyribonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wright P J
DeLucia A L
Tegtmeyer P
References (32)
32 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.12.2631-2638.1984
Published
1984-12-00
Pages
2631-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369271
Subset
IM
Grants
NCI NIH HHS · CA-18808 · United States
NCI NIH HHS · CA-28146 · United States
External Links
PubMed source DOI: 10.1128/mcb.4.12.2631-2638.1984 PMC Full Text View journal details
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