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PMID: 3014503 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transcription of human papillomavirus type 16 early genes in a cervical cancer and a cancer-derived cell line and identification of the E7 protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 13 ·1986-07-00 ·Pages 4680-4

Smotkin D, Wettstein FO

Abstract

Human papillomavirus type 16 DNA and RNA were characterized in the cervical cancer-derived CaSki cell line, which contains only integrated DNA, and in a cervical cancer, which contains predominantly plasmid DNA. In both, a major RNA can code for the early open reading frame E7 and a minor one can code for E6. The cervical cancer, but not the CaSki cell line, contains a minor RNA that can code for an intact E2 protein, and this may relate to the continued presence of plasmid DNA. The RNA mapping data suggest that the poly(A)+ RNA is transcribed from a minor fraction of the several hundred gene copies present, and in the cervical cancer these genomes appear to be integrated. The E7 protein has been identified in CaSki cells and the prevalence of its mRNA suggests a possible function in progression to, or long-term maintenance of, the malignant state.

MeSH Terms
Cell Line Chromosome Mapping DNA, Neoplasm/genetics DNA, Viral/genetics Female Genes, Viral Humans Molecular Weight Papillomaviridae/genetics RNA, Messenger/genetics RNA, Viral/genetics Transcription, Genetic Uterine Cervical Neoplasms/microbiology Viral Proteins/genetics
Chemicals
DNA, Neoplasm DNA, Viral RNA, Messenger RNA, Viral Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Smotkin D
Wettstein F O
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30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.13.4680
Published
1986-07-00
Pages
4680-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323805
Subset
IM
Grants
NCI NIH HHS · CA 18151 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.13.4680 PMC Full Text View journal details
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