Home LiteratureArticle Details
PMID: 3016320 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Transduction of c-src coding and intron sequences by a transformation-defective deletion mutant of Rous sarcoma virus.

Journal of virology ·Vol. 59 ·No. 3 ·1986-09-00 ·Pages 556-63

Soong MM, Iijima S, Wang LH

Abstract

The mechanism of cellular src (c-src) transduction by a transformation-defective deletion mutant, td109, of Rous sarcoma virus was studied by sequence analysis of the recombinational junctions in three td109-derived recovered sarcoma viruses (rASVs). Our results show that two rASVs have been generated by recombination between td109 and c-src at the region between exons 1 and 2 defined previously. Significant homology between td109 and c-src sequences was present at the sites of recombination. The viral and c-src sequence junction of the third rASV was formed by splicing a cryptic donor site at the 5' region of env of td109 to exon 1 of c-src. Various lengths of c-src internal intron 1 sequences were incorporated into all three rASV genomes, which resulted from activation of potential splice donor and acceptor sites. The incorporated intron 1 sequences were absent in the c-src mRNA, excluding its being the precursor for recombination with td109 and implying that initial recombinations most likely took place at the DNA level. A potential splice acceptor site within the incorporated intron 1 sequences in two rASVs was activated and was used for the src mRNA synthesis in infected cells. The normal env mRNA splice acceptor site was used for src mRNA synthesis for the third rASV.

MeSH Terms
Avian Sarcoma Viruses/genetics Base Sequence Cell Transformation, Neoplastic Cell Transformation, Viral Genes, Viral Mutation Oncogenes RNA Splicing RNA, Messenger/genetics,metabolism Recombination, Genetic Sequence Homology, Nucleic Acid
Chemicals
RNA, Messenger
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Soong M M
Iijima S
Wang L H
References (54)
54 references, click to expand
  1. Selective extraction of polyoma DNA from infected mouse cell cultures.
    J Mol Biol. 1967 Jun 14;26(2):365-9 PMID: 4291934
  2. Identification of the viral sequence required for the generation of recovered avian sarcoma viruses and characterization of a series of replication-defective recovered avian sarcoma viruses.
    J Virol. 1984 Mar;49(3):881-91 PMID: 6321772
  3. Properties and location of poly(A) in Rous sarcoma virus RNA.
    J Virol. 1974 Dec;14(6):1515-29 PMID: 4372409
  4. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  5. Rat insulin genes: construction of plasmids containing the coding sequences.
    Science. 1977 Jun 17;196(4296):1313-9 PMID: 325648
  6. Recovery of avian sarcoma virus from tumors induced by transformation-defective mutants.
    J Exp Med. 1977 Dec 1;146(6):1735-47 PMID: 200700
  7. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  8. A new method for the purification and identification of covalently closed circular DNA molcules.
    Nucleic Acids Res. 1978 Apr;5(4):1139-52 PMID: 26042
  9. Recombination between viral and cellular sequences generates transforming sarcoma virus.
    Proc Natl Acad Sci U S A. 1978 Dec;75(12):5812-6 PMID: 215998
  10. Characterization of some isolates of newly recovered avian sarcoma virus.
    J Virol. 1979 Jan;29(1):91-101 PMID: 219237
  11. Restitution of fibroblast-transforming ability in src deletion mutants of avian sarcoma virus during animal passage.
    Virology. 1979 Mar;93(2):413-26 PMID: 222047
  12. Analysis of the src gene of sarcoma viruses generated by recombination between transformation-defective mutants and quail cellular sequences.
    J Virol. 1979 Nov;32(2):546-56 PMID: 228078
  13. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  14. Two mRNAs can be produced from a single immunoglobulin mu gene by alternative RNA processing pathways.
    Cell. 1980 Jun;20(2):313-9 PMID: 6771020
  15. Evidence for the common origin of viral and cellular sequences involved in sarcomagenic transformation.
    J Virol. 1980 Jul;35(1):52-64 PMID: 6251277
  16. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  17. A single mouse alpha-amylase gene specifies two different tissue-specific mRNAs.
    Cell. 1981 Feb;23(2):451-8 PMID: 6162570
  18. Viral and cellular src genes contribute to the structure of recovered avian sarcoma virus transforming protein.
    Cell. 1981 Apr;24(1):155-64 PMID: 6263484
  19. Genetic structure, transforming sequence, and gene product of avian sarcoma virus UR1.
    J Virol. 1981 Oct;40(1):258-67 PMID: 6270378
  20. Molecular cloning of the Fujinami sarcoma virus genome and its comparison with sequences of other related transforming viruses.
    J Virol. 1982 Jun;42(3):1007-16 PMID: 6284986
  21. Nucleotide sequence of Fujinami sarcoma virus: evolutionary relationship of its transforming gene with transforming genes of other sarcoma viruses.
    Cell. 1982 Oct;30(3):787-95 PMID: 6291784
  22. DNA sequence of the viral and cellular src gene of chickens. 1. Complete nucleotide sequence of an EcoRI fragment of recovered avian sarcoma virus which codes for gp37 and pp60src.
    J Virol. 1982 Oct;44(1):1-11 PMID: 6292477
  23. Alternative transcription and two modes of splicing results in two myosin light chains from one gene.
    Nature. 1984 Mar 22-28;308(5957):333-8 PMID: 6709041
  24. Induction of tumors and generation of recovered sarcoma viruses by, and mapping of deletions in, two molecularly cloned src deletion mutants.
    J Virol. 1984 Jun;50(3):904-13 PMID: 6328021
  25. Molecular cloning and characterization of avian sarcoma virus UR2 and comparison of its transforming sequence with those of other avian sarcoma viruses.
    J Virol. 1984 Jun;50(3):914-21 PMID: 6328022
  26. Splicing pathways of SV40 mRNAs in X. laevis oocytes differ in their requirements for snRNPs.
    Cell. 1984 Jul;37(3):927-36 PMID: 6086149
  27. Alternative RNA processing: determining neuronal phenotype.
    Science. 1984 Sep 21;225(4668):1315-20 PMID: 6089345
  28. Nucleic acid sequences of the oncogene v-rel in reticuloendotheliosis virus strain T and its cellular homolog, the proto-oncogene c-rel.
    J Virol. 1984 Oct;52(1):172-82 PMID: 6090694
  29. Mechanisms for the generation of src-deletion mutants and recovered sarcoma viruses: identification of viral sequences involved in src deletions and in recombination with c-src sequences.
    Virology. 1984 Oct 30;138(2):236-45 PMID: 6093366
  30. Rous sarcoma virus encodes a transcriptional activator.
    Cell. 1985 Mar;40(3):537-46 PMID: 2982497
  31. Cloning and nucleotide sequences of cDNAs spanning the splice junctions of Rous sarcoma virus mRNAs.
    J Virol. 1985 Mar;53(3):969-72 PMID: 2579248
  32. Nucleotide sequence and topography of chicken c-fps. Genesis of a retroviral oncogene encoding a tyrosine-specific protein kinase.
    J Mol Biol. 1985 Jan 20;181(2):175-86 PMID: 3879969
  33. Deletion in the 3' pol sequence correlates with aberration of RNA expression in certain replication-defective avian sarcoma viruses.
    J Virol. 1985 May;54(2):446-59 PMID: 2985807
  34. Intricate combinatorial patterns of exon splicing generate multiple regulated troponin T isoforms from a single gene.
    Cell. 1985 May;41(1):67-82 PMID: 2986851
  35. c-erbB activation in ALV-induced erythroblastosis: novel RNA processing and promoter insertion result in expression of an amino-truncated EGF receptor.
    Cell. 1985 Jul;41(3):719-26 PMID: 2988784
  36. Partial nucleotide sequence of Rous sarcoma virus-29 provides evidence that the original Rous sarcoma virus was replication defective.
    J Virol. 1985 Sep;55(3):728-35 PMID: 2991593
  37. Alternative splicing caused by RNA secondary structure.
    Cell. 1985 Dec;43(3 Pt 2):667-76 PMID: 4075405
  38. Alternative splicing accounts for the four forms of myelin basic protein.
    Cell. 1985 Dec;43(3 Pt 2):721-7 PMID: 2416470
  39. Proto-oncogene c-ros codes for a molecule with structural features common to those of growth factor receptors and displays tissue specific and developmentally regulated expression.
    Mol Cell Biol. 1986 May;6(5):1478-86 PMID: 3023892
  40. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
    Gene. 1982 Oct;19(3):269-76 PMID: 6295880
  41. Organization of the rat gamma-fibrinogen gene: alternative mRNA splice patterns produce the gamma A and gamma B (gamma ') chains of fibrinogen.
    Cell. 1982 Nov;31(1):159-66 PMID: 6897622
  42. Drosophila has one myosin heavy-chain gene with three developmentally regulated transcripts.
    Cell. 1983 Jan;32(1):23-34 PMID: 6402306
  43. Alternative RNA splicing of the murine alpha A-crystallin gene: protein-coding information within an intron.
    Cell. 1983 Mar;32(3):707-12 PMID: 6187470
  44. Nucleotide sequence of Rous sarcoma virus.
    Cell. 1983 Mar;32(3):853-69 PMID: 6299578
  45. Structure and sequence of the cellular gene homologous to the RSV src gene and the mechanism for generating the transforming virus.
    Cell. 1983 Mar;32(3):881-90 PMID: 6299580
  46. Analysis of FBJ-MuSV provirus and c-fos (mouse) gene reveals that viral and cellular fos gene products have different carboxy termini.
    Cell. 1983 Apr;32(4):1241-55 PMID: 6301687
  47. Transduction of a cellular oncogene: the genesis of Rous sarcoma virus.
    Proc Natl Acad Sci U S A. 1983 May;80(9):2519-23 PMID: 6302692
  48. Analysis of the pathogenicity of transformation defective partial deletion mutants of avian sarcoma virus: characterization of recovered viruses which encode novel src specific proteins.
    Virology. 1983 Jun;127(2):397-411 PMID: 6306917
  49. Neural tissues express high levels of the cellular src gene product pp60c-src.
    Mol Cell Biol. 1983 Jun;3(6):1157-62 PMID: 6192323
  50. Transduction of c-myb into avian myeloblastosis virus: locating points of recombination within the cellular gene.
    J Virol. 1983 Dec;48(3):565-72 PMID: 6195355
  51. Alternative RNA splicing in expression of the H-2K gene.
    Nature. 1983 Dec 8-14;306(5943):602-4 PMID: 6689056
  52. Three different fibronectin mRNAs arise by alternative splicing within the coding region.
    Cell. 1983 Dec;35(2 Pt 1):421-31 PMID: 6317187
  53. DNA sequence of the Bryan high-titer strain of Rous sarcoma virus: extent of env deletion and possible genealogical relationship with other viral strains.
    J Virol. 1984 Feb;49(2):549-56 PMID: 6319754
  54. Ribonucleic acid isolated by cesium chloride centrifugation.
    Biochemistry. 1974 Jun 4;13(12):2633-7 PMID: 4831907
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.59.3.556-563.1986
Published
1986-09-00
Pages
556-63
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC253207
Subset
IM
Grants
NCI NIH HHS · CA18213 · United States
NCI NIH HHS · CA24930 · United States
NCI NIH HHS · CA29339 · United States
Databases
GENBANK
M13447, M13455, M13456
External Links
PubMed source DOI: 10.1128/JVI.59.3.556-563.1986 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2