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PMID: 6319754 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

DNA sequence of the Bryan high-titer strain of Rous sarcoma virus: extent of env deletion and possible genealogical relationship with other viral strains.

Journal of virology ·Vol. 49 ·No. 2 ·1984-02-00 ·Pages 549-56

Lerner TL, Hanafusa H

Abstract

The genetic structure of the Bryan high-titer strain of Rous sarcoma virus (BH-RSV) was analyzed by using a molecular clone obtained from proviral DNA. DNA sequencing of the pol-src junction of BH-RSV revealed that the env sequence was almost entirely absent; only six base pairs following the pol termination codon remained. Beginning at nucleotide 7 (relative to the end of pol), a 91-base pair sequence identical to the 91 base pairs immediately upstream from src in other strains of RSV was found. The helper virus-related sequence of about 100 base pairs, which is present as a direct repeat in the 5' and 3' regions flanking src in other RSVs, was present only on the 3' side of src in BH-RSV. The 3' end of BH-RSV, from the last 16 base pairs of src through the U3 region, was virtually identical to a region downstream of env through U3 in the nontransforming helper virus Rous-associated virus-2, suggesting that BH-RSV may have been derived by recombination between Rous-associated virus-2 and cellular src DNA. The possibility that the original RSV may have been a defective transforming virus and a precursor of the nondefective RSV strains is discussed.

MeSH Terms
Amino Acid Sequence Animals Avian Sarcoma Viruses/genetics Base Sequence Cell Line Chromosome Deletion Cloning, Molecular DNA Restriction Enzymes DNA, Viral/genetics Genes, Viral Rats Species Specificity Viral Proteins/genetics
Chemicals
DNA, Viral Viral Proteins DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lerner T L
Hanafusa H
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45 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.49.2.549-556.1984
Published
1984-02-00
Pages
549-56
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255496
Subset
IM
Grants
NCI NIH HHS · CA14935 · United States
NCI NIH HHS · CA18213 · United States
NIAID NIH HHS · T32AI07233 · United States
Databases
GENBANK
K03365, K03366
External Links
PubMed source DOI: 10.1128/JVI.49.2.549-556.1984 PMC Full Text View journal details
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