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PMID: 3016478 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Conservation and variation in the large scale organisation of the globin gene domains of duck and chicken.

Molecular & general genetics : MGG ·Vol. 203 ·No. 2 ·1986-05-00 ·Pages 193-201

Kretsovali A, Marcaud L, Moreau J, Scherrer K

Abstract

The genomic DNA of cloned recombinants containing the duck globin genes was compared to that of the analogous domains of the chicken. A 36 kb insert including the three alpha-type globin genes was isolated from a newly prepared duck genomic library in the cosmid PJB8; another recombinant contained a 45 kb insert with the four beta globin genes. In the alpha globin gene domain, the relative positions of genes, of repetitive sequences, and of the A + T-rich segments (AT-rich linkers, ATRLs) which frame the gene cluster (Moreau et al. 1982), were found to be closely maintained between duck and chicken. Although ATRLs and repetitive sequences also frame the gene cluster in the beta globin domains of duck and chicken, there is more genetic drift in their relative positions than in the alpha domain. It is of interest that several repetitive DNA segments were detected in the chicken beta globin domain which do not exist in corresponding positions in the duck. In view of the strict conservation in both species of genes and their relative positions in the cluster, this observation seems to exclude a simple function of repetitive sequences in the control of individual genes. The data are discussed with regard to the possible significance of repetitive and AT-rich DNA segments in genome organisation and function.

MeSH Terms
Animals Chickens Cloning, Molecular Cosmids DNA Restriction Enzymes Ducks Genes Genetic Variation Globins/genetics Nucleic Acid Denaturation Nucleic Acid Hybridization Repetitive Sequences, Nucleic Acid Species Specificity
Chemicals
Globins DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kretsovali A
Marcaud L
Moreau J
Scherrer K
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65 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00333954
Published
1986-05-00
Pages
193-201
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00333954
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