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PMID: 3016483 Published · ppublish English Journal Article

Rearrangements of microinjected recombinant DNA in the genome of transgenic mice.

Molecular & general genetics : MGG ·Vol. 203 ·No. 2 ·1986-05-00 ·Pages 305-11

Tarantul VZ, Kucheriavy VV, Makarova IV, Baranov YuN, Begetova TV, Andreeva LE, Gazaryan KG

Abstract

Previously, mouse zygotes were microinjected with the recombinant plasmid pMA3, which contains the Herpes simplex virus thymidine kinase gene attached to the promoter region of the Rous sarcoma virus (Gazaryan et al. 1984a). In the present work the pMA3 fragment with the flanking genomic sequences was isolated from the DNA of one transgenic Fo mouse by the "plasmid rescue" technique. The rescued plasmid (pMAR1) lacked all virus-specific sequences and retained only some pBR322 sequences. The flanking region at one end of the integrated pBR322-specific fragment contained a highly conserved mouse repetitive sequence. The possible mechanisms of rearrangement of foreign DNA in germ line cells are discussed.

MeSH Terms
Animals DNA Restriction Enzymes DNA, Recombinant/administration & dosage,metabolism Escherichia coli/genetics Genes Genes, Viral Liver/enzymology Mice Mice, Inbred C57BL Microinjections Nucleic Acid Hybridization Plasmids Simplexvirus/enzymology,genetics Thymidine Kinase/genetics
Chemicals
DNA, Recombinant Thymidine Kinase DNA Restriction Enzymes
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Tarantul V Z
Kucheriavy V V
Makarova I V
Baranov YuN
Begetova T V
Andreeva L E
Gazaryan K G
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00333971
Published
1986-05-00
Pages
305-11
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00333971
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