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PMID: 3018513 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

DNA-mediated transfer and expression of a human DNA repair gene that demethylates O6-methylguanine.

Molecular and cellular biology ·Vol. 5 ·No. 11 ·1985-11-00 ·Pages 3293-6

Ding R, Ghosh K, Eastman A, Bresnick E

Abstract

Human liver DNA was transfected into CHO cells (mex-) along with pSV2gpt and colonies were selected first for resistance to mycophenolic acid and then to chloroethylnitrosourea. Transformants were obtained that contained approximately 10,000 molecules of O6-alkylguanine alkyltransferase (mex+) per cell. Their genome contained at least three copies of the human Alu sequence.

MeSH Terms
Animals Cell Line Cricetinae Cricetulus DNA Repair DNA Restriction Enzymes Drug Resistance Ethylnitrosourea/analogs & derivatives,pharmacology Female Genes Guanine/analogs & derivatives,metabolism Humans Liver/metabolism Methyltransferases/genetics Mycophenolic Acid/pharmacology O(6)-Methylguanine-DNA Methyltransferase Ovary Transformation, Genetic
Chemicals
1-(2-chloroethyl)-1-nitrosourea Guanine O-(6)-methylguanine Methyltransferases O(6)-Methylguanine-DNA Methyltransferase DNA Restriction Enzymes Mycophenolic Acid Ethylnitrosourea
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ding R
Ghosh K
Eastman A
Bresnick E
References (22)
22 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.11.3293-3296.1985
Published
1985-11-00
Pages
3293-6
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369147
Subset
IM
Grants
NCI NIH HHS · CA 00906 · United States
NCI NIH HHS · CA 36679 · United States
NCI NIH HHS · CA 36727 · United States
External Links
PubMed source DOI: 10.1128/mcb.5.11.3293-3296.1985 PMC Full Text View journal details
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