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PMID: 3019667 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Studies on the expression of an H-2K/human growth hormone fusion gene in giant transgenic mice.

The EMBO journal ·Vol. 5 ·No. 8 ·1986-08-00 ·Pages 1877-83

Morello D, Moore G, Salmon AM, Yaniv M, Babinet C

Abstract

Transgenic mice carrying the H-2K/human growth hormone (hGH) fusion gene were produced by microinjecting into the pronucleus of fertilized eggs DNA molecules containing 2 kb of the 5' flanking sequences (including promoter) of the class I H-2Kb gene joined to the coding sequences of the hGH gene. Thirteen transgenic mice were obtained which all contained detectable levels of hGH hormone in their blood. Nine grew larger than their control litter-mates. Endogenous H-2Kb and exogenous hGH mRNA levels were analysed by S1 nuclease digestion experiments. hGH transcripts were found in all the tissues examined and the pattern of expression paralleled that of endogenous H-2K gene expression, being high in liver and lymphoid organs and low in muscle and brain. Thus 2 kb of the 5' promoter/regulatory region of the H-2K gene are sufficient to ensure regulated expression of hGH in transgenic mice. This promoter may therefore be of use to target the expression of different exogenous genes in most tissues of transgenic mice and to study the biological role of the corresponding proteins in different cellular environments.

MeSH Terms
Animals Base Sequence DNA Restriction Enzymes Embryo, Mammalian Genes Growth Hormone/genetics H-2 Antigens/genetics Mice Microinjections Nucleic Acid Hybridization RNA, Messenger/genetics
Chemicals
H-2 Antigens H-2Kb protein, mouse RNA, Messenger Growth Hormone DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Morello D
Moore G
Salmon A M
Yaniv M
Babinet C
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29 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-08-00
Pages
1877-83
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1167053
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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