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PMID: 3020371 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Incompatibility between plasmids with independent copy control.

Molecular & general genetics : MGG ·Vol. 204 ·No. 2 ·1986-08-00 ·Pages 341-8

Projan SJ, Novick RP

Abstract

Incompatibility between autonomous plasmids has been attributed, for the most part, to interaction between plasmids' negative control systems and/or partitioning systems. In this report it is shown that indirectly regulated plasmids with non-interactive negative control systems are incompatible on the basis of their shared initiator protein. This principle was demonstrated for a family of Staphylococcus aureus plasmids whose copy number is regulated by inhibitory RNAs that control the production of a rate-limiting, trans-active, initiator protein. We have constructed a pair of plasmids that have the same regulation systems and different initiator proteins and another pair with different regulation systems and the same initiators. Both of these pairs of plasmids were shown to be incompatible.

MeSH Terms

Base Sequence Cloning, Molecular DNA Restriction Enzymes Mutation Plasmids Species Specificity Staphylococcus/genetics Staphylococcus Phages/genetics Transcription, Genetic

Chemicals

DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Projan S J

Novick R P

References (27)

27 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00425520

Published

1986-08-00

Pages

341-8

Language

English

Region

Germany

NLM ID

0125036

Subset

Grants

NIGMS NIH HHS · GM14372 · United States

NIGMS NIH HHS · GM33278 · United States

External Links

PubMed source DOI: 10.1007/BF00425520

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Incompatibility between plasmids with independent copy control.

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