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PMID: 3020504 Published · ppublish English Comparative Study Journal Article

Nucleotide sequence of the tetM tetracycline resistance determinant of the streptococcal conjugative shuttle transposon Tn1545.

Nucleic acids research ·Vol. 14 ·No. 17 ·1986-09-11 ·Pages 7047-58

Martin P, Trieu-Cuot P, Courvalin P

Abstract

The nucleotide sequence of the tetracycline resistance gene tetM encoded by streptococcal conjugative shuttle transposon Tn1545 has been determined. The resistance gene was identified as a coding sequence of 1917 base pairs corresponding to a protein with a Mr of 72,500 daltons. This value is in good agreement with that, 68,000 daltons, estimated by SDS-polyacrylamide gel electrophoresis of Escherichia coli minicell extracts. The tetM gene product does not exhibit any sequence homology with either the Gram-negative (tetA, tetB and tetC), or the Bacillus and Staphylococcus tetracycline resistance proteins. The average hydropathy value of the tetM gene product (-0.21) contrasts with those calculated for the other TET proteins which are markedly hydrophobic (0.76 to 0.93). Hybridization experiments performed with an intragenic tetM probe do not support the claim [Taylor, D. (1986), J. Bact. 165, 1037-1039)] that tetracycline resistance in Campylobacter is due to acquisition of tetM.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Cloning, Molecular Conjugation, Genetic DNA Transposable Elements DNA, Bacterial/genetics Drug Resistance, Microbial Escherichia coli/genetics Genes, Bacterial Solubility Streptococcus/genetics Tetracycline
Chemicals
Bacterial Proteins DNA Transposable Elements DNA, Bacterial Tetracycline
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Martin P
Trieu-Cuot P
Courvalin P
References (44)
44 references, click to expand
  1. Nucleotide sequence of the rightward operator of phage lambda.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8 PMID: 1055375
  2. Susceptibility of ribosomes of the tetracycline-producing strain of Streptomyces aureofaciens to tetracyclines.
    FEBS Lett. 1983 Feb 7;152(1):125-30 PMID: 6404649
  3. Translation of Drosophila melanogaster sequences in Escherichia coli.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):5041-5 PMID: 412196
  4. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  5. Complete nucleotide sequence of the Escherichia coli plasmid pBR322.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:77-90 PMID: 383387
  6. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  7. A new computer method for the storage and manipulation of DNA gel reading data.
    Nucleic Acids Res. 1980 Aug 25;8(16):3673-94 PMID: 7433103
  8. Active efflux of tetracycline encoded by four genetically different tetracycline resistance determinants in Escherichia coli.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3974-7 PMID: 7001450
  9. Evidence for a chromosome-borne resistance transposon (Tn916) in Streptococcus faecalis that is capable of "conjugal" transfer in the absence of a conjugative plasmid.
    J Bacteriol. 1981 Jan;145(1):494-502 PMID: 6257641
  10. A system for shotgun DNA sequencing.
    Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
  11. Heterogeneity of tetracycline resistance determinants in Streptococcus.
    J Bacteriol. 1982 Mar;149(3):995-1004 PMID: 6801018
  12. Closely related plasmids from Staphylococcus aureus and soil bacilli.
    Plasmid. 1982 Mar;7(2):152-62 PMID: 7079389
  13. The making of strand-specific M13 probes.
    Gene. 1982 Mar;17(3):271-7 PMID: 7049837
  14. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  15. Directed mutagenesis method for analysis of mutagen specificity: application to ultraviolet-induced mutagenesis.
    Proc Natl Acad Sci U S A. 1983 Jan;80(1):237-41 PMID: 6337373
  16. Nucleotide sequence of the Tn10 encoded tetracycline resistance gene.
    Nucleic Acids Res. 1983 Jan 25;11(2):525-39 PMID: 6298728
  17. Rapid similarity searches of nucleic acid and protein data banks.
    Proc Natl Acad Sci U S A. 1983 Feb;80(3):726-30 PMID: 6572363
  18. Revised sequence of the tetracycline-resistance gene of pBR322.
    Gene. 1983 May-Jun;22(2-3):277-80 PMID: 6307828
  19. Streptococcus plasmid pAM alpha 1 is a composite of two separable replicons, one of which is closely related to Bacillus plasmid pBC16.
    J Bacteriol. 1983 Aug;155(2):607-15 PMID: 6307973
  20. The tetracycline resistance determinants of RP1 and Tn1721: nucleotide sequence analysis.
    Nucleic Acids Res. 1983 Sep 10;11(17):6089-105 PMID: 6310527
  21. Complete nucleotide sequence of pT181, a tetracycline-resistance plasmid from Staphylococcus aureus.
    Plasmid. 1983 Nov;10(3):251-9 PMID: 6657777
  22. Sequence homology between the tetracycline-resistance determinants of Tn10 and pBR322.
    Gene. 1983 Nov;25(1):83-92 PMID: 6319234
  23. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis.
    Gene. 1983 Dec;26(1):101-6 PMID: 6323249
  24. Characterization and expression of a cloned tetracycline resistance determinant from the chromosome of Streptococcus mutans.
    J Bacteriol. 1984 Nov;160(2):556-63 PMID: 6094475
  25. A conjugative transposon (Tn919) in Streptococcus sanguis.
    Infect Immun. 1985 Feb;47(2):415-20 PMID: 2981772
  26. Structural organization of a 67-kilobase streptococcal conjugative element mediating multiple antibiotic resistance.
    J Bacteriol. 1985 Feb;161(2):620-6 PMID: 3881405
  27. Molecular cloning of tetracycline resistance genes from Streptomyces rimosus in Streptomyces griseus and characterization of the cloned genes.
    J Bacteriol. 1985 Mar;161(3):1010-6 PMID: 2982781
  28. Nucleotide sequence of ermA, a macrolide-lincosamide-streptogramin B determinant in Staphylococcus aureus.
    J Bacteriol. 1985 May;162(2):633-40 PMID: 2985541
  29. The DNA sequence of the gene for the secreted Bacillus subtilis enzyme levansucrase and its genetic control sites.
    Mol Gen Genet. 1985;200(2):220-8 PMID: 2993818
  30. Tetracycline-resistant Mycoplasma hominis strains contain streptococcal tetM sequences.
    Antimicrob Agents Chemother. 1985 Jul;28(1):141-3 PMID: 2994555
  31. Nucleotide sequence of the tetracycline resistance gene of pTHT15, a thermophilic Bacillus plasmid: comparison with staphylococcal TcR controls.
    Gene. 1985;37(1-3):131-8 PMID: 2996983
  32. Structural relationship between the genes encoding 3'-aminoglycoside phosphotransferases in Campylobacter and in gram-positive cocci.
    Ann Inst Pasteur Microbiol. 1985 Sep-Oct;136B(2):135-50 PMID: 3002237
  33. Streptococcal tetracycline resistance mediated at the level of protein synthesis.
    J Bacteriol. 1986 Feb;165(2):564-9 PMID: 3080409
  34. In vivo transfer of genetic information between gram-positive and gram-negative bacteria.
    EMBO J. 1985 Dec 16;4(13A):3583-7 PMID: 3937729
  35. A complementation analysis of the restriction and modification of DNA in Escherichia coli.
    J Mol Biol. 1969 May 14;41(3):459-72 PMID: 4896022
  36. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  37. Improved estimation of secondary structure in ribonucleic acids.
    Nat New Biol. 1973 Nov 14;246(150):40-1 PMID: 4519026
  38. Identification of two plasmids determining resistance to tetracycline and to erythromycin in group D streptococcus.
    Mol Gen Genet. 1974;132(3):181-92 PMID: 4214420
  39. Plasmid-mediated tetracycline resistance in Campylobacter jejuni: expression in Escherichia coli and identification of homology with streptococcal class M determinant.
    J Bacteriol. 1986 Mar;165(3):1037-9 PMID: 3005233
  40. Nucleotide sequence and organization of Bacillus subtilis RNA polymerase major sigma (sigma 43) operon.
    Nucleic Acids Res. 1986 May 27;14(10):4293-307 PMID: 3086839
  41. Dissemination of the tetM tetracycline resistance determinant to Ureaplasma urealyticum.
    Antimicrob Agents Chemother. 1986 Feb;29(2):350-2 PMID: 3521481
  42. Studies of antimicrobial resistance genes using DNA probes.
    Antimicrob Agents Chemother. 1986 May;29(5):721-5 PMID: 3524426
  43. Heterogeneity of tetracycline resistance determinants.
    Plasmid. 1980 Mar;3(2):99-108 PMID: 6927764
  44. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.17.7047
Published
1986-09-11
Pages
7047-58
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311716
Subset
IM
Databases
GENBANK
X04388
External Links
PubMed source DOI: 10.1093/nar/14.17.7047 PMC Full Text View journal details
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