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PMID: 3023912 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Saccharomyces cerevisiae contains two functional citrate synthase genes.

Molecular and cellular biology ·Vol. 6 ·No. 6 ·1986-06-00 ·Pages 1936-42

Kim KS, Rosenkrantz MS, Guarente L

Abstract

The tricarboxylic acid cycle occurs within the mitochondria of the yeast Saccharomyces cerevisiae. A nuclear gene encoding the tricarboxylic acid cycle enzyme citrate synthase has previously been isolated (M. Suissa, K. Suda, and G. Schatz, EMBO J. 3:1773-1781, 1984) and is referred to here as CIT1. We report here the isolation, by an immunological method, of a second nuclear gene encoding citrate synthase (CIT2). Disruption of both genes in the yeast genome was necessary to produce classical citrate synthase-deficient phenotypes: glutamate auxotrophy and poor growth on rich medium containing lactate, a nonfermentable carbon source. Therefore, the citrate synthase produced from either gene was sufficient for these metabolic roles. Transcription of both genes was maximally repressed in medium containing both glucose and glutamate. However, transcription of CIT1 but not of CIT2 was derepressed in medium containing a nonfermentable carbon source. The significance of the presence of two genes encoding citrate synthase in S. cerevisiae is discussed.

MeSH Terms
Chromosome Mapping Citrate (si)-Synthase/genetics DNA Restriction Enzymes Gene Expression Regulation Genes, Fungal Genetic Complementation Test Glucose/physiology Glutamates/physiology Lactates/physiology Oxo-Acid-Lyases/genetics RNA, Messenger/genetics Saccharomyces cerevisiae/genetics Transcription, Genetic
Chemicals
Glutamates Lactates RNA, Messenger Citrate (si)-Synthase DNA Restriction Enzymes Oxo-Acid-Lyases Glucose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kim K S
Rosenkrantz M S
Guarente L
References (32)
32 references, click to expand
  1. Effect of different nutritional conditions on the synthesis of tricarboxylic acid cycle enzymes.
    J Bacteriol. 1967 Jun;93(6):1777-87 PMID: 4960893
  2. Changes in the enzyme activities of Saccharomyces cerevisiae during aerobic growth on different carbon sources.
    Biochem J. 1965 Oct;97(1):284-97 PMID: 16749116
  3. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
  4. Nucleotide sequence of yeast LEU2 shows 5'-noncoding region has sequences cognate to leucine.
    Cell. 1982 Dec;31(2 Pt 1):319-25 PMID: 6297759
  5. Import of proteins into mitochondria. Partial purification of a matrix-located protease involved in cleavage of mitochondrial precursor polypeptides.
    J Biol Chem. 1983 Apr 25;258(8):4937-43 PMID: 6300104
  6. Coarse and fine control of citrate synthase from Bacillus subtilis.
    Biochim Biophys Acta. 1969 Jul 30;184(2):252-62 PMID: 4980242
  7. Citrate synthase.
    Curr Top Cell Regul. 1976;10:161-204 PMID: 3389
  8. Isolation and characterization of tricarboxylic acid cycle mutants of Bacillus subtilis.
    J Bacteriol. 1971 Jun;106(3):848-55 PMID: 4997541
  9. Transformation in Escherichia coli: cryogenic preservation of competent cells.
    J Bacteriol. 1977 Oct;132(1):349-51 PMID: 334731
  10. Structure and function of the yeast URA3 gene: expression in Escherichia coli.
    Gene. 1984 Jul-Aug;29(1-2):113-24 PMID: 6092217
  11. Derepression of citrate synthase in Saccharomyces cerevisiae may occur at the level of transcription.
    Mol Cell Biol. 1984 Feb;4(2):247-53 PMID: 6199662
  12. Crystallographic refinement and atomic models of two different forms of citrate synthase at 2.7 and 1.7 A resolution.
    J Mol Biol. 1982 Jun 15;158(1):111-52 PMID: 7120407
  13. A rapid boiling method for the preparation of bacterial plasmids.
    Anal Biochem. 1981 Jun;114(1):193-7 PMID: 6269464
  14. Studies on the regulation and localization of the glyoxylate cycle enzymes in Saccharomyces cerevisiae.
    Eur J Biochem. 1969 Aug;10(1):83-9 PMID: 5345986
  15. Intracellular localization of enzymes in yeast.
    Arch Biochem Biophys. 1970 Jan;136(1):245-59 PMID: 4391967
  16. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  17. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
    Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052
  18. One-step gene disruption in yeast.
    Methods Enzymol. 1983;101:202-11 PMID: 6310324
  19. In vitro translation of mRNA for yeast citrate synthase.
    J Biol Chem. 1982 Sep 25;257(18):11181-5 PMID: 6809768
  20. Distinctly regulated tandem upstream activation sites mediate catabolite repression of the CYC1 gene of S. cerevisiae.
    Cell. 1984 Feb;36(2):503-11 PMID: 6319028
  21. Citrate synthaseless glutamic acid auxotroph of Saccharomyces cerevisiae.
    Mol Gen Genet. 1975 Sep 8;139(4):303-9 PMID: 1102943
  22. Bacillus subtilis citB gene is regulated synergistically by glucose and glutamine.
    J Bacteriol. 1985 Oct;164(1):155-64 PMID: 2413006
  23. Regulation of the level of yeasts citrate synthase by oxygen availability.
    Mol Cell Biochem. 1976 Sep 30;12(3):161-9 PMID: 790160
  24. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  25. Characterization of rate-controlling steps in vivo by use of an adjustable expression vector.
    Proc Natl Acad Sci U S A. 1985 Jun;82(11):3577-81 PMID: 3889909
  26. Yeast RNA polymerase II genes: isolation with antibody probes.
    Science. 1983 Nov 18;222(4625):778-82 PMID: 6356359
  27. A GAL10-CYC1 hybrid yeast promoter identifies the GAL4 regulatory region as an upstream site.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7410-4 PMID: 6760197
  28. Heme regulates transcription of the CYC1 gene of S. cerevisiae via an upstream activation site.
    Cell. 1983 Apr;32(4):1279-86 PMID: 6301690
  29. Yeast promoters and lacZ fusions designed to study expression of cloned genes in yeast.
    Methods Enzymol. 1983;101:181-91 PMID: 6310321
  30. Extramitochondrial citrate synthase activity in bakers' yeast.
    Mol Cell Biol. 1986 Feb;6(2):488-93 PMID: 3023850
  31. Isolation of the nuclear yeast genes for citrate synthase and fifteen other mitochondrial proteins by a new screening method.
    EMBO J. 1984 Aug;3(8):1773-81 PMID: 6090126
  32. Transformation of intact yeast cells treated with alkali cations.
    J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.6.1936-1942.1986
Published
1986-06-00
Pages
1936-42
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367731
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.6.6.1936-1942.1986 PMC Full Text View journal details
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