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PMID: 3024107 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Splice site consensus sequences are preferentially accessible to nucleases in isolated adenovirus RNA.

Nucleic acids research ·Vol. 14 ·No. 21 ·1986-11-11 ·Pages 8447-65

Munroe SH, Duthie RS

Abstract

The conformation of RNA sequences spanning five 3' splice sites and two 5' splice sites in adenovirus mRNA was probed by partial digestion with single-strand specific nucleases. Although cleavage of nucleotides near both 3' and 5' splice sites was observed, most striking was the preferential digestion of sequences near the 3' splice site. At each 3' splice site a region of very strong cleavage is observed at low concentrations of enzyme near the splice site consensus sequence or the upstream branch point consensus sequence. Additional sites of moderately strong cutting near the branch point consensus sequence were observed in those sequences where the splice site was the preferred target. Since recognition of the 3' splice site and branch site appear to be early events in mRNA splicing these observations may indicate that the local conformation of the splice site sequences may play a direct or indirect role in enhancing the accessibility of sequences important for splicing.

MeSH Terms
Adenovirus Early Proteins Adenoviruses, Human/genetics Base Sequence DNA Restriction Enzymes Genes Genes, Viral Nucleic Acid Conformation Oncogene Proteins, Viral/genetics Plasmids RNA Splicing RNA, Viral/genetics Serotyping Transcription, Genetic
Chemicals
Adenovirus Early Proteins Oncogene Proteins, Viral RNA, Viral DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Munroe S H
Duthie R S
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47 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.21.8447
Published
1986-11-11
Pages
8447-65
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311870
Subset
IM
Grants
NCRR NIH HHS · RR07196 · United States
External Links
PubMed source DOI: 10.1093/nar/14.21.8447 PMC Full Text View journal details
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