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PMID: 6095200 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Secondary structure of splice sites in adenovirus mRNA precursors.

Nucleic acids research ·Vol. 12 ·No. 22 ·1984-11-26 ·Pages 8437-56

Munroe SH

Abstract

In order to investigate the possible role of RNA secondary structure in determining the efficiency and specificity of mRNA splicing, the structures of sequences at three acceptor splice sites in adenovirus were studied. Transcripts spanning intron-exon junctions were synthesized using SP6 RNA polymerase and analyzed using single and double-strand specific nucleases. Distinctive patterns of nuclease cleavage were observed for each of the 3 sites examined. At both sites in the E2a region sequences adjacent to the splice sites were particularly susceptible to digestion with T1 and S1 nucleases. In contrast, a splice site for hexon mRNA was largely resistant to these nucleases. The results obtained suggest that the conformation of the RNA at some, but not all, acceptor sites may enhance the accessibility of these sites to factors involved in splicing nuclear RNA and confirm the presence of a large, previously predicted hairpin structure centered on the acceptor site at 67 map units.

MeSH Terms
Adenoviruses, Human/genetics Base Sequence DNA Restriction Enzymes Genes, Viral Genetic Vectors Nucleic Acid Conformation Nucleic Acid Precursors/genetics Plasmids RNA Precursors RNA Splicing RNA, Messenger/genetics Repetitive Sequences, Nucleic Acid Templates, Genetic Transcription, Genetic
Chemicals
Nucleic Acid Precursors RNA Precursors RNA, Messenger DNA Restriction Enzymes
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Munroe S H
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38 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.22.8437
Published
1984-11-26
Pages
8437-56
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320384
Subset
IM
Grants
NCRR NIH HHS · RR07196 · United States
External Links
PubMed source DOI: 10.1093/nar/12.22.8437 PMC Full Text View journal details
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