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PMID: 3025558 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

An upstream signal is required for in vitro transcription of Neurospora 5S RNA genes.

Molecular & general genetics : MGG ·Vol. 205 ·No. 1 ·1986-10-00 ·Pages 189-92

Selker EU, Morzycka-Wroblewska E, Stevens JN, Metzenberg RL

Abstract

The DNA sequences upstream of the 5S RNA genes in Neurospora crassa are largely different from one another, but share a short consensus sequence located in the segment 29 to 26 nucleotides preceding the transcribed region. Differences among flanking sequences do not appear to affect transcription. Deletion analysis indicates, however, that a DNA segment including the conserved "TATA box" is required for in vitro transcription of Neurospora 5S RNA genes.

MeSH Terms
Base Sequence DNA Restriction Enzymes Genes, Fungal Neurospora/genetics Promoter Regions, Genetic RNA, Ribosomal/genetics Transcription, Genetic
Chemicals
RNA, Ribosomal DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Selker E U
Morzycka-Wroblewska E
Stevens J N
Metzenberg R L
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31 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF02428052
Published
1986-10-00
Pages
189-92
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM-08995 · United States
NIGMS NIH HHS · GM-35690 · United States
External Links
PubMed source DOI: 10.1007/BF02428052
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