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PMID: 3025617 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Structural and functional analysis of the MAL1 locus of Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 6 ·No. 11 ·1986-11-00 ·Pages 3891-9

Charron MJ, Dubin RA, Michels CA

Abstract

We describe the isolation of a 22.6-kilobase fragment of DNA containing the MAL1 locus of Saccharomyces cerevisiae. Our results demonstrate that the MAL1 locus, like the MAL6 locus, is a complex locus containing three genes. These genes were organized similarly to their MAL6 counterparts. We refer to them as MAL11, MAL12, and MAL13 and show that they are functionally homologous to the MAL61 (encoding maltose permease), MAL62 (encoding maltase), and MAL63 (encoding the positive regulator) genes of the MAL6 locus. Transcription from each of the three genes was analyzed in a strain carrying the undisrupted MAL1 locus and in strains carrying single disruptions in each of the MAL1 genes. The MAL1 and MAL1 loci were found to be highly sequence homologous and conserved throughout the region containing these three genes. The strain used to isolate the MAL1 locus also carried the tightly linked SUC1 gene. The SUC1 gene was found to be located on the same 22.6-kilobase fragment containing the MAL1 locus and 5 kilobases from the 3' end of the MAL12 gene. The meaning of these results with regard to the mechanism of regulation of maltose fermentation is discussed.

MeSH Terms
Chromosome Deletion Cloning, Molecular DNA Restriction Enzymes Genes Genes, Fungal Genes, Regulator Genotype Membrane Transport Proteins/genetics Monosaccharide Transport Proteins Mutation Plasmids Saccharomyces cerevisiae/genetics Transcription, Genetic alpha-Glucosidases/genetics
Chemicals
Membrane Transport Proteins Monosaccharide Transport Proteins maltose permease DNA Restriction Enzymes alpha-Glucosidases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Charron M J
Dubin R A
Michels C A
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26 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.11.3891-3899.1986
Published
1986-11-00
Pages
3891-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367152
Subset
IM
Grants
NIGMS NIH HHS · GM-28216 · United States
External Links
PubMed source DOI: 10.1128/mcb.6.11.3891-3899.1986 PMC Full Text View journal details
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