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PMID: 3027698 Published · ppublish English Journal Article

Amino acid sequence of S-adenosyl-L-homocysteine hydrolase from rat liver as derived from the cDNA sequence.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 3 ·1987-02-00 ·Pages 719-23

Ogawa H, Gomi T, Mueckler MM, Fujioka M, Backlund PS, Aksamit RR, Unson CG, Cantoni GL

Abstract

Rat liver cDNA libraries constructed in lambda gt11 were screened for reactivity with polyclonal antibodies to native S-adenosyl-L-homocysteine (AdoHcy) hydrolase (adenosylhomocysteinase; EC 3.3.1.1). Five clones were isolated and sequenced. The amino acid sequence, deduced from the cDNA sequence, contained the sequence of eight peptides obtained by tryptic and cyanogen bromide fragmentation of rat liver AdoHcy hydrolase. Identification of the amino- and carboxyl-terminal peptides in the amino acid sequence showed that the complete sequence was obtained. A "fingerprint" sequence was found that is characteristic of dinucleotide-binding domains of many proteins. For AdoHcy hydrolase, this region from the lysine at position 213 to the aspartate at position 244, containing the sequence Gly-Xaa-Gly-Xaa-Xaa-Gly at positions 219-224, is presumably the site of binding for NAD+, which is required for the activity of the enzyme.

MeSH Terms
Adenosylhomocysteinase Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA/analysis DNA Restriction Enzymes Hydrolases/genetics Liver/enzymology Peptide Fragments/analysis Rats Sequence Homology, Nucleic Acid
Chemicals
Peptide Fragments DNA Hydrolases DNA Restriction Enzymes Adenosylhomocysteinase
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Ogawa H
Gomi T
Mueckler M M
Fujioka M
Backlund P S
Aksamit R R
Unson C G
Cantoni G L
References (47)
47 references, click to expand
  1. Succinylation of pepsinogen.
    J Biol Chem. 1967 Jun 10;242(11):2739-45 PMID: 5338505
  2. The enzymatic synthesis of S-adenosyl-L-homocysteine from adenosine and homocysteine.
    J Biol Chem. 1959 Mar;234(3):603-8 PMID: 13641268
  3. Thermodynamics of nicotinamide-adenine dinucleotide addition to the glyceraldehyde 3-phosphate dehydrogenases of yeast and of rabbit skeletal muscle. An equilibrium and calorimetric analysis over a range of temperatures.
    Biochemistry. 1971 Mar 2;10(5):779-86 PMID: 5544669
  4. Mechanism for enzymatic thioether formation. Mechanism of action of S-adenosylhomocysteinase.
    J Biol Chem. 1976 Sep 25;251(18):5817-9 PMID: 965391
  5. Amino acid sequence of dogfish muscle lactate dehydrogenase.
    J Biol Chem. 1977 Mar 10;252(5):1799-806 PMID: 838743
  6. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  7. Adenosylhomocysteine hydrolase. Crystallization of the purified enzyme and its properties.
    J Biol Chem. 1978 Jun 25;253(12):4476-80 PMID: 659427
  8. S-adenosylhomocysteine hydrolase is an adenosine-binding protein: a target for adenosine toxicity.
    Science. 1978 Nov 17;202(4369):757-60 PMID: 715439
  9. An adenosine 3':5'-monophosphate adenosine-binding protein from mouse liver. Association with S-adenosylhomocysteinase activity.
    FEBS Lett. 1978 Dec 1;96(1):125-8 PMID: 215449
  10. The mechanism of action of S-adenosylhomocysteinase.
    J Biol Chem. 1979 Feb 25;254(4):1217-26 PMID: 762125
  11. Guanine nucleotide-binding activity as an assay for src protein of rat-derived murine sarcoma viruses.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):5355-9 PMID: 228288
  12. Purification and characterization of two high-affinity (adenosine 3',5'-monophosphate)-binding proteins from yeast. Identification as multiple forms of glyceraldehyde-3-phosphate dehydrogenase.
    Eur J Biochem. 1980 Aug;109(1):39-49 PMID: 6250837
  13. The ligand specificity of the (adenosine 3',5'-monophosphate)-binding site of yeast glyceraldehyde-3-phosphate dehydrogenase. Interaction with adenosine derivatives and pharmacologically-active compounds.
    Eur J Biochem. 1980 Aug;109(1):51-9 PMID: 6997047
  14. Exons encode functional and structural units of chicken lysozyme.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):5759-63 PMID: 6934509
  15. S-Adenosylhomocysteine hydrolase from rat liver. Purification and some properties.
    J Biol Chem. 1981 Feb 25;256(4):1631-5 PMID: 7462216
  16. Multiple polyadenylation sites in a mouse alpha-amylase gene.
    Nucleic Acids Res. 1981 May 25;9(10):2313-23 PMID: 6166922
  17. Affinity-chromatographic purification of S-adenosyl-L-homocysteine hydrolase. Some properties of the enzyme from rat liver.
    Biochem J. 1981 Feb 1;193(2):503-12 PMID: 7305945
  18. Glutathione reductase from human erythrocytes. The sequences of the NADPH domain and of the interface domain.
    Eur J Biochem. 1982 Jan;121(2):259-67 PMID: 7060551
  19. The human genes for S-adenosylhomocysteine hydrolase and adenosine deaminase are syntenic on chromosome 20.
    Science. 1982 May 14;216(4547):739-42 PMID: 7079734
  20. S-Adenosylhomocysteinase: mechanism of inactivation by 2'-deoxyadenosine and interaction with other nucleosides.
    Biochemistry. 1982 Oct 26;21(22):5557-62 PMID: 6983366
  21. Comparison of some physicochemical and kinetic properties of S-adenosylhomocysteine hydrolase from bovine liver, bovine adrenal cortex and mouse liver.
    Biochim Biophys Acta. 1982 Nov 9;708(2):185-93 PMID: 6293581
  22. Homology between human bladder carcinoma oncogene product and mitochondrial ATP-synthase.
    Nature. 1983 Jan 20;301(5897):262-4 PMID: 6296696
  23. Predicted nucleotide-binding properties of p21 protein and its cancer-associated variant.
    Nature. 1983 Apr 28;302(5911):842-4 PMID: 6843652
  24. The pathway of eukaryotic mRNA formation.
    Annu Rev Biochem. 1983;52:441-66 PMID: 6193753
  25. Yeast RNA polymerase II genes: isolation with antibody probes.
    Science. 1983 Nov 18;222(4625):778-82 PMID: 6356359
  26. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.
    Nucleic Acids Res. 1984 Jan 25;12(2):857-72 PMID: 6694911
  27. Amino acid analysis by reverse-phase high-performance liquid chromatography: precolumn derivatization with phenylisothiocyanate.
    Anal Biochem. 1984 Jan;136(1):65-74 PMID: 6711815
  28. Occurrence of S-adenosylhomocysteine hydrolase in prokaryote cells. Characterization of the enzyme from Alcaligenes faecalis and role of the enzyme in the activated methyl cycle.
    Eur J Biochem. 1984 Jun 1;141(2):385-92 PMID: 6428887
  29. Direct evidence that oncogenic tyrosine kinases and cyclic AMP-dependent protein kinase have homologous ATP-binding sites.
    Nature. 1984 Aug 16-22;310(5978):589-92 PMID: 6431300
  30. Human adenosine deaminase. cDNA and complete primary amino acid sequence.
    J Biol Chem. 1984 Oct 10;259(19):12101-6 PMID: 6090454
  31. Chromogenic immunodetection of human serum albumin and alpha-L-fucosidase clones in a human hepatoma cDNA expression library.
    DNA. 1984 Dec;3(6):437-47 PMID: 6096099
  32. Sequence of the initiation factor IF2 gene: unusual protein features and homologies with elongation factors.
    Proc Natl Acad Sci U S A. 1984 Dec;81(24):7787-91 PMID: 6096856
  33. S-Adenosylhomocysteinase from rat liver. Evidence for structurally identical and catalytically equivalent subunits.
    J Biol Chem. 1985 Mar 10;260(5):2789-93 PMID: 3972804
  34. Human insulin receptor and its relationship to the tyrosine kinase family of oncogenes.
    Nature. 1985 Feb 28-Mar 6;313(6005):756-61 PMID: 2983222
  35. Rapid and sensitive protein similarity searches.
    Science. 1985 Mar 22;227(4693):1435-41 PMID: 2983426
  36. Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family.
    Nucleic Acids Res. 1985 Mar 11;13(5):1431-42 PMID: 2987824
  37. GTPase of bovine rod outer segments: the amino acid sequence of the alpha subunit as derived from the cDNA sequence.
    Proc Natl Acad Sci U S A. 1985 Jul;82(13):4316-20 PMID: 2989813
  38. Identification of functional murine adenosine deaminase cDNA clones by complementation in Escherichia coli.
    J Biol Chem. 1985 Aug 25;260(18):10299-307 PMID: 2410423
  39. Inactivation of S-adenosyl-L-homocysteine hydrolase by cAMP results from dissociation of enzyme-bound NAD+.
    Proc Natl Acad Sci U S A. 1985 Jul;82(14):4578-81 PMID: 2991881
  40. Sequence of the precursor to rat ornithine aminotransferase deduced from a cDNA clone.
    J Biol Chem. 1985 Oct 25;260(24):12993-7 PMID: 3840476
  41. Cloning and expression of the human erythropoietin gene.
    Proc Natl Acad Sci U S A. 1985 Nov;82(22):7580-4 PMID: 3865178
  42. Lysine residue 121 in the proposed ATP-binding site of the v-mos protein is required for transformation.
    Proc Natl Acad Sci U S A. 1985 Dec;82(23):7894-8 PMID: 2999782
  43. Isolation and characterization of cDNA for chicken muscle adenylate kinase.
    J Biol Chem. 1986 Feb 25;261(6):2942-5 PMID: 2419325
  44. The complete primary structure of protein kinase C--the major phorbol ester receptor.
    Science. 1986 Aug 22;233(4766):853-9 PMID: 3755547
  45. S-adenosylhomocysteinase from rat liver. Amino acid sequences of the peptides containing active site cysteine residues modified by treatment with 5'-p-fluorosulfonylbenzoyladenosine.
    J Biol Chem. 1986 Oct 15;261(29):13422-5 PMID: 3759971
  46. S-adenosylhomocysteinase: mechanism of reversible and irreversible inactivation by ATP, cAMP, and 2'-deoxyadenosine.
    Biochemistry. 1986 Dec 16;25(25):8337-42 PMID: 3028476
  47. Horse liver alcohol dehydrogenase. The primary structure of the protein chain of the ethanol-active isoenzyme.
    Eur J Biochem. 1970 Sep;16(1):25-40 PMID: 4917230
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.3.719
Published
1987-02-00
Pages
719-23
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC304287
Subset
IM
Databases
GENBANK
M15185
External Links
PubMed source DOI: 10.1073/pnas.84.3.719 PMC Full Text View journal details
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