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PMID: 3030736 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Illegitimate recombination occurs between the replication origin of the plasmid pC194 and a progressing replication fork.

The EMBO journal ·Vol. 5 ·No. 13 ·1986-12-20 ·Pages 3691-6

Michel B, Ehrlich SD

Abstract

Hybrids between plasmids pC194, pBR322 and the bacteriophage f1 undergo deletions in Escherichia coli. The deletions end most often between nucleotides 1445 and 1446 of pC194. That site probably corresponds to a nick in the replication origin of this plasmid. The localization of the other deletion end appears to be determined by the position of the f1 replication fork. Two models accounting for these data are discussed.

MeSH Terms
Bacillus subtilis/genetics Base Sequence Chromosome Deletion DNA Replication DNA Restriction Enzymes Escherichia coli/genetics Genes, Bacterial Plasmids Recombination, Genetic
Chemicals
DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Michel B
Ehrlich S D
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37 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-12-20
Pages
3691-6
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1167412
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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