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PMID: 3031013 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular cloning, characterization, and chromosomal localization of dapF, the Escherichia coli gene for diaminopimelate epimerase.

Journal of bacteriology ·Vol. 169 ·No. 4 ·1987-04-00 ·Pages 1454-9

Richaud C, Higgins W, Mengin-Lecreulx D, Stragier P

Abstract

The Escherichia coli dapF gene was isolated from a cosmid library as a result of screening for clones overproducing diaminopimelate epimerase. Insertional mutagenesis was performed on the cloned dapF gene with a mini-Mu transposon, leading to chloramphenicol resistance. One of these insertions was transferred onto the chromosome by a double-recombination event, allowing us to obtain a dapF mutant. This mutant accumulated large amounts of LL-diaminopimelate, confirming the blockage in the step catalyzed by the dapF product, but did not require meso-diaminopimelate for growth. The dapF gene was localized in the 85-min region of the E. coli chromosome between cya and uvrD.

MeSH Terms
Amino Acid Isomerases Chromosome Mapping Chromosomes, Bacterial Cloning, Molecular DNA Transposable Elements Diaminopimelic Acid/metabolism Escherichia coli/enzymology,genetics Genes, Bacterial Isomerases/genetics Mutation Racemases and Epimerases/genetics
Chemicals
DNA Transposable Elements Diaminopimelic Acid Isomerases Racemases and Epimerases Amino Acid Isomerases diaminopimelate epimerase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Richaud C
Higgins W
Mengin-Lecreulx D
Stragier P
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29 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.4.1454-1459.1987
Published
1987-04-00
Pages
1454-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211967
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.169.4.1454-1459.1987 PMC Full Text View journal details
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