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PMID: 3033271 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Herpes simplex virus infection generates large tandemly reiterated simian virus 40 DNA molecules in a transformed hamster cell line.

Journal of virology ·Vol. 61 ·No. 5 ·1987-05-00 ·Pages 1427-34

Matz B

Abstract

When the simian virus 40 (SV40)-transformed Syrian hamster cell line Elona is infected with herpes simplex virus type 1, an excessive amplification of SV40-specific DNA sequences occurs. Analysis of total DNA from herpes simplex virus-infected cells revealed that amplified DNA sequences were present predominantly in a high-molecular-weight form, consisting of a tandem array of many unit-length SV40 DNA molecules. Repeat units of amplified DNA were found to be very similar to standard SV40 DNA as was shown by restriction analyses, except for a small deletion close to the origin of replication, which could also be detected in the chromosomal DNA of uninfected cells. A procedure, devised for selective enrichment of amplified SV40 DNA molecules from the bulk of cellular and herpesviral DNA, allowed molecular cloning of single repeat units and nucleotide sequence analysis of the relative genomic region.

MeSH Terms
Animals Base Sequence Cell Line Cell Transformation, Viral Chromosome Mapping DNA Replication DNA Restriction Enzymes DNA, Viral/genetics Gene Amplification Gene Expression Regulation Herpes Simplex/microbiology Molecular Weight Simian virus 40/genetics Simplexvirus/genetics
Chemicals
DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Matz B
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52 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.61.5.1427-1434.1987
Published
1987-05-00
Pages
1427-34
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC254119
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.61.5.1427-1434.1987 PMC Full Text View journal details
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