Home LiteratureArticle Details
PMID: 3038530 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Primary structure of the gene for the murine Ia antigen-associated invariant chains (Ii). An alternatively spliced exon encodes a cysteine-rich domain highly homologous to a repetitive sequence of thyroglobulin.

The EMBO journal ·Vol. 6 ·No. 6 ·1987-06-00 ·Pages 1677-83

Koch N, Lauer W, Habicht J, Dobberstein B

Abstract

The gene for murine Ia-associated invariant (Ii) chains (Ii31 and Ii41) was characterized by sequence analysis. The gene extends over approximately 9 kb and is organized in nine exons. Exon 1 encodes the 5' untranslated region and the cytoplasmic segment, exon 2 the membrane spanning segment and adjacent amino acids and exons 3-8 the extracytoplasmic portion of Ii31. Putative promoter sequences were found upstream of the start of the coding sequence. Between exons 6 and 7 an additional, alternatively spliced exon 6b has been identified. This exon is spliced into the mRNA coding for the Ii-related Ii41 protein. Exon 6b encodes a cysteine-rich domain of 64 amino acids. It shows a remarkably high homology to the repetitive elements in thyroglobulin, a precursor for thyroid hormone. Based on this homology, it is suggested that this domain (TgR) in Tg and in Ii41 may play a role either in hormone formation or as a carrier in the transport of molecules (thyroid hormone or processed antigen respectively) between intracellular compartments.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cysteine DNA Restriction Enzymes Exons Genes Genes, MHC Class II Histocompatibility Antigens Class II/analysis Mice RNA Splicing Sequence Homology, Nucleic Acid Thyroglobulin/genetics
Chemicals
Histocompatibility Antigens Class II Thyroglobulin DNA Restriction Enzymes Cysteine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Koch N
Lauer W
Habicht J
Dobberstein B
References (63)
63 references, click to expand
  1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  2. Three distinct signals can induce class II gene expression in a murine pre-B-cell line.
    Proc Natl Acad Sci U S A. 1986 Jul;83(13):4878-82 PMID: 3487783
  3. Domains and the hinge region of an immunoglobulin heavy chain are encoded in separate DNA segments.
    Nature. 1979 Feb 22;277(5698):627-33 PMID: 106304
  4. Detection of a common polypeptide chain in I--A and I--E sub-region immunoprecipitates.
    Mol Immunol. 1979 Jan;16(1):51-60 PMID: 376435
  5. Control of thyroglobulin synthesis and secretion. (First of two parts).
    N Engl J Med. 1979 Aug 2;301(5):239-49 PMID: 221813
  6. A subcloning strategy for DNA sequence analysis.
    Nucleic Acids Res. 1980 Dec 11;8(23):5541-9 PMID: 6258146
  7. Primary structural: analysis of the transplantation antigens of the murine H-2 major histocompatibility complex.
    Annu Rev Biochem. 1981;50:1025-52 PMID: 7023355
  8. Organization and expression of eucaryotic split genes coding for proteins.
    Annu Rev Biochem. 1981;50:349-83 PMID: 6791577
  9. Biosynthesis of the human transferrin receptor in cultured cells.
    J Biol Chem. 1981 Dec 25;256(24):12888-92 PMID: 6273413
  10. Membrane insertion and oligomeric assembly of HLA-DR histocompatibility antigens.
    Cell. 1982 May;29(1):61-9 PMID: 6955026
  11. Biosynthesis and glycosylation of the invariant chain associated with HLA-DR antigens.
    J Immunol. 1982 Dec;129(6):2564-9 PMID: 6982931
  12. Detection of two distinct class II alpha:beta:Ii complexes in the Syrian hamster.
    Immunogenetics. 1982;16(5):425-33 PMID: 6961101
  13. Biochemical characterization of an invariant polypeptide associated with Ia antigens in human and mouse.
    Mol Immunol. 1983 Jan;20(1):21-32 PMID: 6574312
  14. Association of human gamma chain with class II transplantation antigens during intracellular transport.
    Biochemistry. 1983 Jun 21;22(13):3206-13 PMID: 6576808
  15. Regulation of MHC class II invariant chain expression: induction of synthesis in human and murine plasmocytoma cells by arresting replication.
    EMBO J. 1983;2(6):811-6 PMID: 6416833
  16. Alternative RNA splicing in expression of the H-2K gene.
    Nature. 1983 Dec 8-14;306(5943):602-4 PMID: 6689056
  17. Differential expression of the invariant chain in mouse tumor cells: relationship to B lymphoid development.
    J Immunol. 1984 Jan;132(1):12-5 PMID: 6418793
  18. Invariant proteins associated with guinea-pig Ia antigens.
    Mol Immunol. 1983 Dec;20(12):1333-45 PMID: 6581383
  19. Ia antigens and associated invariant chain are induced simultaneously in lines of T-dependent mast cells by recombinant interferon-gamma.
    J Immunol. 1984 Mar;132(3):1361-9 PMID: 6420464
  20. A human Ia cytoplasmic determinant located on multiple forms of invariant chain (gamma, gamma 2, gamma 3).
    J Immunol. 1984 Apr;132(4):1900-5 PMID: 6199417
  21. cDNA clone for the human invariant gamma chain of class II histocompatibility antigens and its implications for the protein structure.
    Proc Natl Acad Sci U S A. 1983 Dec;80(24):7395-9 PMID: 6324166
  22. Monensin prevents terminal glycosylation of the N- and O-linked oligosaccharides of the HLA-DR-associated invariant chain and inhibits its dissociation from the alpha-beta chain complex.
    Proc Natl Acad Sci U S A. 1984 Mar;81(5):1287-91 PMID: 6608726
  23. Structure of the murine Ia-associated invariant (Ii) chain as deduced from a cDNA clone.
    EMBO J. 1984 Apr;3(4):873-7 PMID: 6327293
  24. The invariant chains of mouse class II antigens: biochemical properties and molecular relationship.
    Eur J Immunol. 1984 Jun;14(6):511-7 PMID: 6428920
  25. The gene encoding the human class II antigen-associated gamma chain is located on chromosome 5.
    Immunogenetics. 1984;20(1):89-93 PMID: 6746029
  26. Immune interferon activates multiple class II major histocompatibility complex genes and the associated invariant chain gene in human endothelial cells and dermal fibroblasts.
    Proc Natl Acad Sci U S A. 1984 Aug;81(15):4917-21 PMID: 6431411
  27. Primary structure of human transferrin receptor deduced from the mRNA sequence.
    Nature. 1984 Oct 18-24;311(5987):675-8 PMID: 6090955
  28. The human LDL receptor: a cysteine-rich protein with multiple Alu sequences in its mRNA.
    Cell. 1984 Nov;39(1):27-38 PMID: 6091915
  29. Alternative splicing in the 5' moiety of the H-2Kd gene transcript.
    EMBO J. 1984 Oct;3(10):2383-6 PMID: 6094182
  30. Pathways of endocytosis in thyroid follicle cells.
    Int Rev Cytol. 1984;91:107-39 PMID: 6389418
  31. Multiple specific contacts between a mammalian transcription factor and its cognate promoters.
    Nature. 1984 Nov 29-Dec 5;312(5993):409-13 PMID: 6095100
  32. opa: a novel family of transcribed repeats shared by the Notch locus and other developmentally regulated loci in D. melanogaster.
    Cell. 1985 Jan;40(1):55-62 PMID: 2981631
  33. The gene encoding the Ia-associated invariant chain is located on chromosome 18 in the mouse.
    Immunogenetics. 1985;21(1):83-90 PMID: 2981770
  34. HLA-D region antigen-associated invariant polypeptides as revealed by two-dimensional gel analysis. Glycosylation and structural inter-relationships.
    J Biol Chem. 1985 Feb 10;260(3):1927-36 PMID: 2981878
  35. Mouse prepro-epidermal growth factor synthesis by the kidney and other tissues.
    Nature. 1985 Jan 17-23;313(5999):228-31 PMID: 3871506
  36. Sequence of the 5'-end quarter of the human-thyroglobulin messenger ribonucleic acid and of its deduced amino-acid sequence.
    Eur J Biochem. 1985 Feb 15;147(1):53-8 PMID: 3971976
  37. Presence of hormonogenic and repetitive domains in the first 930 amino acids of bovine thyroglobulin as deduced from the cDNA sequence.
    Eur J Biochem. 1985 Feb 15;147(1):59-64 PMID: 3855750
  38. Rapid and sensitive protein similarity searches.
    Science. 1985 Mar 22;227(4693):1435-41 PMID: 2983426
  39. One gene encodes two distinct Ia-associated invariant chains.
    J Immunol. 1985 May;134(5):3461-7 PMID: 3920321
  40. In search of a function for the invariant chain associated with Ia antigens.
    Surv Immunol Res. 1985;4(1):27-34 PMID: 3890055
  41. Structure, expression and regulation of the thyroglobulin gene.
    Mol Cell Endocrinol. 1985 May;40(2-3):89-97 PMID: 3891458
  42. Primary structure of bovine thyroglobulin deduced from the sequence of its 8,431-base complementary DNA.
    Nature. 1985 Aug 15-21;316(6029):647-51 PMID: 3855243
  43. Characterization of the gene encoding the major rat liver asialoglycoprotein receptor.
    J Biol Chem. 1985 Oct 15;260(23):12523-7 PMID: 2995379
  44. Chloroquine affects biosynthesis of Ia molecules by inhibiting dissociation of invariant (gamma) chains from alpha-beta dimers in B cells.
    J Exp Med. 1985 Oct 1;162(4):1371-6 PMID: 3930653
  45. Antigen-presenting function of the macrophage.
    Annu Rev Immunol. 1984;2:395-428 PMID: 6242349
  46. Intracellular class II HLA antigens are accessible to transferrin-neuraminidase conjugates internalized by receptor-mediated endocytosis.
    Proc Natl Acad Sci U S A. 1985 Dec;82(23):8188-92 PMID: 2999796
  47. Differential expression of Ia and Ia-associated invariant chain in mouse tissues after in vivo treatment with IFN-gamma.
    J Immunol. 1986 Feb 1;136(3):940-8 PMID: 3079804
  48. Structure of the human gene encoding the invariant gamma-chain of class II histocompatibility antigens.
    Nucleic Acids Res. 1985 Dec 20;13(24):8827-41 PMID: 3001652
  49. The complete structure of the rat thyroglobulin gene.
    Proc Natl Acad Sci U S A. 1986 Jan;83(2):323-7 PMID: 3455768
  50. A computer program package for restriction map analysis and manipulation.
    Nucleic Acids Res. 1986 Jan 10;14(1):335-49 PMID: 3003676
  51. The HLA-D-associated invariant chain binds palmitic acid at the cysteine adjacent to the membrane segment.
    J Biol Chem. 1986 Mar 5;261(7):3434-40 PMID: 3512557
  52. Analysis of cosmids using linearization by phage lambda terminase.
    Gene. 1985;40(2-3):259-66 PMID: 3007292
  53. A new method of DNA sequencing using deoxynucleoside alpha-thiotriphosphates.
    DNA. 1986 Apr;5(2):173-7 PMID: 3709339
  54. T3 delta pre-mRNA is transcribed from a non-TATA promoter and is alternatively spliced in human T cells.
    EMBO J. 1986 Jun;5(6):1245-52 PMID: 3488209
  55. Efficient cell surface expression of class II MHC molecules in the absence of associated invariant chain.
    J Exp Med. 1986 Nov 1;164(5):1478-89 PMID: 3464691
  56. Cell surface expression of class II histocompatibility antigens occurs in the absence of the invariant chain.
    J Exp Med. 1986 Nov 1;164(5):1490-504 PMID: 2430042
  57. Two forms of the Ia antigen-associated invariant chain result from alternative initiations at two in-phase AUGs.
    Cell. 1986 Nov 21;47(4):619-25 PMID: 3779839
  58. Induction of metallothionein and other mRNA species by carcinogens and tumor promoters in primary human skin fibroblasts.
    Mol Cell Biol. 1986 May;6(5):1760-6 PMID: 3785178
  59. Alternative splicing and alternative initiation of translation explain the four forms of the Ia antigen-associated invariant chain.
    EMBO J. 1986 Dec 20;5(13):3483-8 PMID: 3104027
  60. Signal recognition particle-dependent membrane insertion of mouse invariant chain: a membrane-spanning protein with a cytoplasmically exposed amino terminus.
    J Cell Biol. 1986 Jun;102(6):2169-75 PMID: 3458708
  61. Structure of the human Ia-associated invariant (gamma)-chain gene: identification of 5' sequences shared with major histocompatibility complex class II genes.
    Proc Natl Acad Sci U S A. 1986 Jun;83(12):4484-8 PMID: 3459184
  62. Analysis of sequence and structure homologies between thyroglobulin and acetylcholinesterase: possible functional and clinical significance.
    Biochem Biophys Res Commun. 1986 May 29;137(1):142-8 PMID: 3718507
  63. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-06-00
Pages
1677-83
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553541
Subset
IM
Databases
GENBANK
X05428, X05429, X05430
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2