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PMID: 3040669 Published · ppublish English Journal Article

Structural and genetic analyses of a par locus that regulates plasmid partition in Bacillus subtilis.

Journal of bacteriology ·Vol. 169 ·No. 9 ·1987-09-00 ·Pages 3952-62

Chang S, Chang SY, Gray O

Abstract

The Bacillus plasmid pLS11 partitions faithfully during cell division. Using a partition-deficient plasmid vector, we randomly cloned DNA fragments of plasmid pLS11 and identified the locus that regulates plasmid partition (par) by cis complementation in Bacillus subtilis. The cloned par gene conferred upon the vector plasmid a high degree of segregational stability. The par locus was mapped to a 167-base-pair segment on pLS11, and its nucleotide sequence was determined. The cloned par fragment regulated the partition of several different Bacillus replicons, and it only functioned in cis; it did not contain the replication function nor elevate the plasmid copy number in B. subtilis. The expression of par was orientation specific with respect to the replication origin on the same plasmid. We propose that the pLS11-derived par functions as a single-stranded site that interacts with other components involved in plasmid partition during cell division.

MeSH Terms
Bacillus subtilis/cytology,genetics Base Sequence Cell Division Chromosome Mapping Cloning, Molecular DNA Replication DNA Restriction Enzymes DNA, Bacterial/analysis Gene Expression Regulation Genes, Bacterial Genetic Complementation Test Phenotype Plasmids Sequence Homology, Nucleic Acid
Chemicals
DNA, Bacterial DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Chang S
Chang S Y
Gray O
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33 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.9.3952-3962.1987
Published
1987-09-00
Pages
3952-62
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC213693
Subset
IM
Databases
GENBANK
M17344, M17345, M17346
External Links
PubMed source DOI: 10.1128/jb.169.9.3952-3962.1987 PMC Full Text View journal details
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