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PMID: 6290448 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Replication and incompatibility properties of plasmid pE194 in Bacillus subtilis.

Journal of bacteriology ·Vol. 152 ·No. 2 ·1982-11-00 ·Pages 722-35

Gryczan TJ, Hahn J, Contente S, Dubnau D

Abstract

pE194, a 3.5-kilobase multicopy plasmid, confers resistance to the macrolide-lincosamide-streptogramin B antibiotics in Bacillus subtilis. By molecular cloning and deletion analysis we have identified a replication segment on the physical map of this plasmid, which consists of about 900 to 1,000 base pairs. This segment contains the replication origin. It also specifies a trans-acting function (rep) required for the stable replication of pE194 and a negatively acting copy control function which is the product of the cop gene. The target sites for the rep and cop gene products are also within this region. Two incompatibility determinants have been mapped on the pE194 genome and their properties are described. One (incA) resides within the replication region and may be identical to cop. incB, not located in the replication region, expresses incompatibility toward a copy control mutant (cop-6) but not toward the wild-type replicon.

MeSH Terms
Bacillus subtilis/genetics Base Composition Cloning, Molecular DNA Replication DNA Restriction Enzymes Genes, Bacterial Genotype Molecular Weight Plasmids
Chemicals
DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gryczan T J
Hahn J
Contente S
Dubnau D
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33 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.152.2.722-735.1982
Published
1982-11-00
Pages
722-35
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC221522
Subset
IM
Grants
NIAID NIH HHS · AI-10311 · United States
NIAID NIH HHS · AI-17472 · United States
External Links
PubMed source DOI: 10.1128/jb.152.2.722-735.1982 PMC Full Text View journal details
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