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PMID: 3043195 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Bent DNA functions as a replication enhancer in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 8 ·No. 7 ·1988-07-00 ·Pages 2763-9

Williams JS, Eckdahl TT, Anderson JN

Abstract

Previous studies have demonstrated that bent DNA is a conserved property of Saccharomyces cerevisiae autonomously replicating sequences (ARSs). Here we showed that bending elements are contained within ARS subdomains identified by others as replication enhancers. To provide a direct test for the function of this unusual structure, we analyzed the ARS activity of plasmids that contained synthetic bent DNA substituted for the natural bending element in yeast ARS1. The results demonstrated that deletion of the natural bending locus impaired ARS activity which was restored to a near wild-type level with synthetic bent DNA. Since the only obvious common features of the natural and synthetic bending elements are the sequence patterns that give rise to DNA bending, the results suggest that the bent structure per se is crucial for ARS function.

MeSH Terms
Base Sequence DNA/analysis DNA Replication Enhancer Elements, Genetic Genes, Synthetic Mutation Saccharomyces cerevisiae/genetics Structure-Activity Relationship
Chemicals
DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Williams J S
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.
Eckdahl T T
Anderson J N
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.7.2763-2769.1988
Published
1988-07-00
Pages
2763-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363493
Subset
IM
Grants
NCI NIH HHS · 1 PO1 CA36761-01A1 · United States
External Links
PubMed source DOI: 10.1128/mcb.8.7.2763-2769.1988 PMC Full Text View journal details
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