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PMID: 3043198 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

cis-acting translational effects of the 5' noncoding region of c-myc mRNA.

Molecular and cellular biology ·Vol. 8 ·No. 7 ·1988-07-00 ·Pages 2875-83

Parkin N, Darveau A, Nicholson R, Sonenberg N

Abstract

We have previously shown that the 5' noncoding region of mouse c-myc mRNA has a negative effect on translational efficiency in a rabbit reticulocyte lysate (A. Darveau, J. Pelletier, and N. Sonenberg, Proc. Natl. Acad. Sci. USA 82:2315-2319, 1985). We wanted to localize and characterize the inhibitory translational element(s) in the mRNA and to study its effect in other in vitro and in vivo systems. Here we report that the restrictive element is confined to a 240-nucleotide sequence of the 5' noncoding region of mouse c-myc mRNA and that this sequence acts in cis to inhibit the translation of a heterologous mRNA. In addition, we report that the cis-inhibitory effect is also exhibited in microinjected Xenopus oocytes and wheat-germ extracts but not in HeLa cell extracts. Transfection of corresponding plasmid DNA constructs into several established cell lines did not produce the cis-inhibitory effect. A model to explain these results is presented.

MeSH Terms
Animals Cell Line Chromosome Deletion Exons Mice Protein Biosynthesis Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins c-myc Proto-Oncogenes RNA, Messenger/metabolism Regulatory Sequences, Nucleic Acid
Chemicals
Proto-Oncogene Proteins Proto-Oncogene Proteins c-myc RNA, Messenger
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Parkin N
Department of Biochemistry, McGill University, Montreal, Quebec, Canada.
Darveau A
Nicholson R
Sonenberg N
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.7.2875-2883.1988
Published
1988-07-00
Pages
2875-83
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363507
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.8.7.2875-2883.1988 PMC Full Text View journal details
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