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PMID: 3072474 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Expression of human adenosine deaminase in murine hematopoietic cells.

Molecular and cellular biology ·Vol. 8 ·No. 12 ·1988-12-00 ·Pages 5116-25

Belmont JW, MacGregor GR, Wager-Smith K, Fletcher FA, Moore KA, Hawkins D, Villalon D, Chang SM, Caskey CT

Abstract

Multiple replication-defective retrovirus vectors were tested for their ability to transfer and express human adenosine deaminase in vitro and in vivo in a mouse bone marrow transplantation model. High-titer virus production was obtained from vectors by using both a retrovirus long terminal repeat promoter and internal transcriptional units with human c-fos and herpes virus thymidine kinase promoters. After infection of primary murine bone marrow with one of these vectors, human adenosine deaminase was detected in 60 to 85% of spleen colony-forming units and in the blood of 14 of 14 syngeneic marrow transplant recipients. This system offers the opportunity to assess methods for increasing efficiency of gene transfer, for regulation of expression of foreign genes in hematopoietic progenitors, and for long-term measurement of the stability of expression in these cells.

MeSH Terms
Adenosine Deaminase/genetics Animals Blotting, Southern Bone Marrow Cells Bone Marrow Transplantation Cell Line Cells, Cultured Genes Genetic Vectors Hematopoietic Stem Cells/cytology,enzymology Humans Mice Mice, Inbred C57BL Nucleoside Deaminases/genetics Retroviridae/genetics Transfection
Chemicals
Nucleoside Deaminases Adenosine Deaminase
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Belmont J W
Institute for Molecular Genetics, Baylor College of Medicine, Houston, Texas 77030.
MacGregor G R
Wager-Smith K
Fletcher F A
Moore K A
Hawkins D
Villalon D
Chang S M
Caskey C T
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.12.5116-5125.1988
Published
1988-12-00
Pages
5116-25
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365613
Subset
IM
Grants
NICHD NIH HHS · HD21452 · United States
External Links
PubMed source DOI: 10.1128/mcb.8.12.5116-5125.1988 PMC Full Text View journal details
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