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PMID: 3088544 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Nuclear factors bind to regulatory regions of the mouse kappa immunoglobulin gene.

Nucleic acids research ·Vol. 14 ·No. 12 ·1986-06-25 ·Pages 4837-48

Hromas R, Van Ness B

Abstract

At least two regions of the mouse kappa immunoglobulin gene are necessary for appropriate transcription. One is located within the J-C intron; another is located 5' of the transcription start site in all variable region gene segments and contains a highly conserved octanucleotide sequence, ATTTGCAT. Trans-acting nuclear factors have been thought to interact with these regions on the basis of studies with transient transfection of modified immunoglobulin genes into lymphoid and fibroblast cell lines. Using a sensitive gel electrophoresis DNA binding assay, we have found nuclear factors that bind to these two regulatory regions of the kappa gene. Two patterns of specific binding of nuclear factors to DNA sequences containing the J-C intron enhancer were observed. One pattern was seen in cell lines which do not express immunoglobulin, while a different pattern was observed in cell lines which actively transcribe light chain genes. We also find factors which bind to the octanucleotide containing 5' sequence which are distinct from those which bind the J-C enhancer region.

MeSH Terms
Animals Base Sequence Cell Nucleus/physiology Cloning, Molecular DNA-Binding Proteins/metabolism Enhancer Elements, Genetic Gene Expression Regulation Genes, Regulator Immunoglobulin Constant Regions/genetics Immunoglobulin kappa-Chains/genetics Mice Transcription Factors/genetics
Chemicals
DNA-Binding Proteins Immunoglobulin Constant Regions Immunoglobulin kappa-Chains Transcription Factors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hromas R
Van Ness B
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29 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.12.4837
Published
1986-06-25
Pages
4837-48
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311495
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/14.12.4837 PMC Full Text View journal details
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