Home LiteratureArticle Details
PMID: 3088545 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Replication of phage phi 29 DNA in vitro: role of the viral protein p6 in initiation and elongation.

Nucleic acids research ·Vol. 14 ·No. 12 ·1986-06-25 ·Pages 4923-37

Blanco L, Gutiérrez J, Lázaro JM, Bernad A, Salas M

Abstract

The phi 29 protein p6 stimulates the formation of the protein p3-dAMP initiation complex when added to a minimal system containing the terminal protein p3, the phi 29 DNA polymerase p2 and phi 29 DNA-protein p3 complex, by decreasing about 5 fold the Km value for dATP. In addition, protein p6 stimulates elongation of the p3-dAMP initiation complex. Whereas the effect of protein p6 on initiation is similar with protein p3-containing fragments from the right or left phi 29 DNA ends, the stimulation of elongation is higher with the right than with the left phi 29 DNA terminal fragment, suggesting DNA sequence specificity. The stimulation by protein p6 of the initiation and elongation steps of phi 29 DNA replication does not require the presence of the parental protein p3 at the phi 29 DNA ends. No effect of protein p6 was obtained on the elongation of the template-primer poly(dT)-(dA) 12-18 by the phi 29 DNA polymerase.

MeSH Terms
Bacillus subtilis/genetics Bacteriophages/genetics Base Sequence DNA Replication DNA, Viral/genetics DNA-Directed DNA Polymerase/metabolism Deoxyadenine Nucleotides/metabolism Templates, Genetic Viral Proteins/genetics
Chemicals
DNA, Viral Deoxyadenine Nucleotides Viral Proteins DNA-Directed DNA Polymerase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Blanco L
Gutiérrez J
Lázaro J M
Bernad A
Salas M
References (27)
27 references, click to expand
  1. Temperature-sensitive mutants affected in DNA synthesis in phage phi29 of Bacillus subtilis.
    Eur J Biochem. 1972 Dec 4;31(2):367-71 PMID: 4630506
  2. Location of the serine residue involved in the linkage between the terminal protein and the DNA of phage phi 29.
    Nucleic Acids Res. 1985 Nov 11;13(21):7715-28 PMID: 3934646
  3. Bacillus subtilis phage phi29. Characterization of gene products and functions.
    Eur J Biochem. 1976 JUL 1;66(2):229-41 PMID: 820555
  4. Analysis of gene function of bacteriophage phi 29 of Bacillus subtilis: identification of cistrons essential for viral assembly.
    J Virol. 1976 Aug;19(2):501-17 PMID: 822175
  5. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
    J Mol Biol. 1977 Feb 15;110(1):119-46 PMID: 845942
  6. Characterization of a protein covalently linked to the 5' termini of the DNA of Bacillus subtilis phage phi29.
    J Mol Biol. 1978 Feb 25;119(2):269-91 PMID: 416224
  7. RNA polymerase binding sites and transcription map of the DNA of Bacillus subtilis phage phi29.
    J Mol Biol. 1979 Feb 5;127(4):411-36 PMID: 107317
  8. Structure of replicating DNA molecules of Bacillus subtilis bacteriophage phi 29.
    J Virol. 1980 Apr;34(1):187-99 PMID: 6768899
  9. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  10. The protein covalently linked to the 5' termini of the DNA of Bacillus subtilis phage phi 29 is involved in the initiation of DNA replication.
    Virology. 1980 Jul 15;104(1):84-96 PMID: 6771916
  11. DNA replication of bacteriophage phi 29: characterization of the intermediates and location of the termini of replication.
    Virology. 1980 Jul 30;104(2):323-38 PMID: 7395108
  12. Protein p3 is linked to the DNA of phage phi 29 through a phosphoester bond between serine and 5'-dAMP.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6425-8 PMID: 6779279
  13. Nucleotide sequences at the termini of phi 29 DNA.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1336-40 PMID: 6262789
  14. Nucleotide sequence at the termini of the DNA of Bacillus subtilis phage phi 29.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1446-50 PMID: 6262800
  15. Structure of protein-containing replicative intermediates of Bacillus subtilis phage phi 29 DNA.
    Virology. 1982 Jan 15;116(1):1-18 PMID: 6801848
  16. In vitro complex formation between bacteriophage phi 29 terminal protein and deoxynucleotide.
    Biochem Biophys Res Commun. 1982 Apr 14;105(3):1031-6 PMID: 6807309
  17. Nucleotide sequence of the major early region of bacteriophage phi 29.
    Gene. 1982 Mar;17(3):323-35 PMID: 6809534
  18. Initiation of phage phi 29 DNA replication in vitro: formation of a covalent complex between the terminal protein, p3, and 5'-dAMP.
    Proc Natl Acad Sci U S A. 1982 Sep;79(18):5522-6 PMID: 6813861
  19. Cloning and expression in Escherichia coli of the gene coding for the protein linked to the ends of Bacillus subtilis phage phi 29 DNA.
    Gene. 1983 Jan-Feb;21(1-2):65-76 PMID: 6301951
  20. Template requirements for initiation of phage phi 29 DNA replication in vitro.
    Proc Natl Acad Sci U S A. 1984 Jan;81(1):80-4 PMID: 6320176
  21. Purification in a functional form of the terminal protein of Bacillus subtilis phage phi 29.
    Proc Natl Acad Sci U S A. 1984 Mar;81(6):1639-43 PMID: 6424120
  22. Characterization and purification of a phage phi 29-encoded DNA polymerase required for the initiation of replication.
    Proc Natl Acad Sci U S A. 1984 Sep;81(17):5325-9 PMID: 6433348
  23. Replication of bacteriophage phi 29 DNA in vitro: the roles of terminal protein and DNA polymerase.
    Proc Natl Acad Sci U S A. 1984 Sep;81(17):5374-8 PMID: 6433349
  24. Overproduction and purification of protein P6 of Bacillus subtilis phage phi 29: role in the initiation of DNA replication.
    Nucleic Acids Res. 1985 May 10;13(9):3083-100 PMID: 3158884
  25. Replication of phage phi 29 DNA with purified terminal protein and DNA polymerase: synthesis of full-length phi 29 DNA.
    Proc Natl Acad Sci U S A. 1985 Oct;82(19):6404-8 PMID: 3863101
  26. Nucleotide sequence of the major early region of Bacillus subtilis phage PZA, a close relative of phi 29.
    Gene. 1985;38(1-3):45-56 PMID: 3934048
  27. Suppressor-sensitive mutants and genetic map of Bacillus subtilis bacteriophage phi 29.
    Virology. 1974 Nov;62(1):1-16 PMID: 4214301
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.12.4923
Published
1986-06-25
Pages
4923-37
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311501
Subset
IM
Grants
NIGMS NIH HHS · 5 R01 GM27242-06 · United States
External Links
PubMed source DOI: 10.1093/nar/14.12.4923 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2