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PMID: 3097076 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

cDNA cloning of human plasminogen activator-inhibitor from endothelial cells.

The Journal of clinical investigation ·Vol. 78 ·No. 6 ·1986-12-00 ·Pages 1673-80

Ginsburg D, Zeheb R, Yang AY, Rafferty UM, Andreasen PA, Nielsen L, Dano K, Lebo RV, Gelehrter TD

Abstract

Full-length cDNA for plasminogen activator inhibitor (PAI-1) was isolated from a human umbilical vein endothelial cell (HUVEC) lambda gt11 cDNA library. Three overlapping clones were identified by immunologic screening of 10(6) recombinant phage using a rabbit anti-human fibrosarcoma PAI-1 antiserum. The fusion proteins encoded by these three clones also react strongly with a monoclonal mouse anti-human fibrosarcoma PAI-1 antibody. By nucleotide sequence analysis, PAI-1 cDNA encodes a protein containing 402 amino acids with a predicted, nonglycosylated molecular mass of 45 kD. Identity of this material as authentic PAI-1 was confirmed by the presence of high level homology with the primary amino acid sequence of an internal peptide prepared from purified rat hepatoma PAI-1. The predicted amino acid sequence also reveals extensive homology with other members of the serine protease inhibitor gene family. Cultured HUVECs contain two PAI-1 mRNA species, both encoded by a single gene, differing by 1 kb in the 3' untranslated region. The PAI-1 gene is located on human chromosome 7.

MeSH Terms
Amino Acid Sequence Base Sequence Chromosome Mapping Cloning, Molecular Codon DNA/analysis Endothelium/analysis Glycoproteins/analysis,genetics,immunology Humans Plasminogen Activators/antagonists & inhibitors Plasminogen Inactivators RNA, Messenger/analysis
Chemicals
Codon Glycoproteins Plasminogen Inactivators RNA, Messenger DNA Plasminogen Activators
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Ginsburg D
Zeheb R
Yang A Y
Rafferty U M
Andreasen P A
Nielsen L
Dano K
Lebo R V
Gelehrter T D
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI112761
Published
1986-12-00
Pages
1673-80
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC423941
Subset
IM
Grants
NCI NIH HHS · CA-07783 · United States
NCI NIH HHS · CA-22720 · United States
Databases
GENBANK
M16006
External Links
PubMed source DOI: 10.1172/JCI112761 PMC Full Text View journal details
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