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PMID: 3102948 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Extinction of expression of immunoglobulin genes in myeloma X fibroblast somatic cell hybrids.

Molecular and cellular biology ·Vol. 7 ·No. 2 ·1987-02-00 ·Pages 936-9

Greenberg A, Ber R, Kra-Oz Z, Laskov R

Abstract

Adherent hybrids between immunoglobulin-producing mouse myeloma cells and fibroblasts do not produce immunoglobulin polypeptide chains. These hybrids retained the actively rearranged immunoglobulin genes of the myeloma parental cells but lacked immunoglobulin heavy- and light-chain RNA transcripts. We conclude that the shutoff of immunoglobulin production in these hybrids occurs at the transcription or early processing level.

MeSH Terms
Animals Fibroblasts/physiology Gene Expression Regulation Hybrid Cells/physiology Immunoglobulin gamma-Chains/genetics Immunoglobulin kappa-Chains/genetics Immunoglobulins/genetics Mice Plasmacytoma/physiopathology RNA, Messenger/genetics Transcription, Genetic
Chemicals
Immunoglobulin gamma-Chains Immunoglobulin kappa-Chains Immunoglobulins RNA, Messenger
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Greenberg A
Ber R
Kra-Oz Z
Laskov R
References (38)
38 references, click to expand
  1. Suppression of immunoglobulin synthesis by cellular hybridization.
    Nat New Biol. 1971 May 19;231(20):87-90 PMID: 4103301
  2. Expression of differentiated functions in hepatoma cell hybrids. I. Tyrosine aminotransferase in hepatoma-fibroblast hybrids.
    Proc Natl Acad Sci U S A. 1971 Jan;68(1):127-31 PMID: 4395684
  3. Cloning of mouse myeloma cells and detection of rare variants.
    J Cell Physiol. 1972 Jun;79(3):429-40 PMID: 4625122
  4. Expression of differentiated functions in hepatoma cell hybrids. VI. Extinction and re-expression of liver alcohol dehydrogenase.
    Biochimie. 1972;54(2):195-201 PMID: 4343793
  5. Use of somatic cell hybrids for analysis of the differentiated state.
    Bacteriol Rev. 1973 Jun;37(2):197-214 PMID: 4581783
  6. Gene expression in somatic cell hybrids.
    Annu Rev Genet. 1974;8:195-218 PMID: 4613257
  7. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  8. Expression of differentiated functions in hepatoma cell hybrids: IX extinction and reexpression of liver-specific enzymes in rat hepatoma-Chinese hamster fibroblast hybrids.
    Somatic Cell Genet. 1975 Jan;1(1):27-40 PMID: 17164
  9. Gene activation in somatic nuclei after injection into amphibian oocytes.
    Proc Natl Acad Sci U S A. 1977 Jun;74(6):2470-4 PMID: 267940
  10. Regulation of immunoglobulin expression in mouse myeloma cells.
    Cold Spring Harb Symp Quant Biol. 1977;41 Pt 2:781-91 PMID: 408077
  11. Immunoglobulin light-chain structural gene sequences cloned in a bacterial plasmid.
    Nature. 1978 Feb 9;271(5645):582-5 PMID: 414143
  12. Rescue of immunoglobulin secretion from human neoplastic lymphoid cells by somatic cell hybridization.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2411-5 PMID: 97669
  13. The synthesis and processing of the messenger RNAs specifying heavy and light chain immunoglobulins in MPC-11 cells.
    Cell. 1978 Dec;15(4):1495-509 PMID: 103631
  14. Induction of amplified synthesis and secretion of IgM by fusion of murine 'b lymphoma with myeloma cells.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):915-9 PMID: 311476
  15. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  16. An unusual translocation of immunoglobulin gene segments in variants of the mouse myeloma MPC11.
    Nature. 1980 Jul 10;286(5769):170-3 PMID: 6250042
  17. RNA splicing generates a variant light chain from an aberrantly rearranged kappa gene.
    Nature. 1980 Aug 21;286(5775):776-9 PMID: 6772972
  18. A mutant immunoglobulin light chain is formed by aberrant DNA- and RNA-splicing events.
    Nature. 1980 Aug 21;286(5775):779-83 PMID: 6772973
  19. Production of monoclonal antibodies: strategy and tactics.
    J Immunol Methods. 1980;35(1-2):1-21 PMID: 7009747
  20. Analysis of immunoglobulin mRNA in murine myeloma cell variants defective in the synthesis of the light or heavy polypeptide chains.
    J Immunol. 1982 Feb;128(2):684-90 PMID: 6798120
  21. On immunoglobulin heavy chain gene switching: two gamma 2b genes are rearranged via switch sequences in MPC-11 cells but only one is expressed.
    Nucleic Acids Res. 1982 Jan 22;10(2):611-30 PMID: 6278424
  22. Isolation and characterization of rat skeletal muscle and cytoplasmic actin genes.
    Proc Natl Acad Sci U S A. 1982 May;79(9):2763-7 PMID: 6953429
  23. DNA rearrangements in MPC-11 immunoglobulin heavy chain class-switch variants.
    Proc Natl Acad Sci U S A. 1982 May;79(9):3006-10 PMID: 6806822
  24. Continuing kappa-gene rearrangement in a cell line transformed by Abelson murine leukemia virus.
    Cell. 1982 Oct;30(3):807-16 PMID: 6291785
  25. A tissue-specific transcription enhancer element is located in the major intron of a rearranged immunoglobulin heavy chain gene.
    Cell. 1983 Jul;33(3):717-28 PMID: 6409417
  26. A lymphocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain genes.
    Cell. 1983 Jul;33(3):729-40 PMID: 6409418
  27. Immunoglobulin gene transcription is activated by downstream sequence elements.
    Cell. 1983 Jul;33(3):741-8 PMID: 6409419
  28. Cell-specific expression controlled by the 5'-flanking region of insulin and chymotrypsin genes.
    Nature. 1983 Dec 8-14;306(5943):557-61 PMID: 6358900
  29. Repression of rearranged mu gene and translocated c-myc in mouse 3T3 cells X Burkitt lymphoma cell hybrids.
    Science. 1984 Apr 27;224(4647):399-402 PMID: 6424234
  30. Time course of arrest of immunoglobulin expression in heterokaryons and early hybrids of human lymphoma cells and mouse fibroblasts. A study of transcriptional and translational events.
    Exp Cell Res. 1985 Jun;158(2):349-59 PMID: 3924638
  31. Control of eukaryotic messenger RNA synthesis by sequence-specific DNA-binding proteins.
    Nature. 1985 Aug 29-Sep 4;316(6031):774-8 PMID: 4041012
  32. DELETION OF THYMIDINE KINASE ACTIVITY FROM L CELLS RESISTANT TO BROMODEOXYURIDINE.
    Exp Cell Res. 1963 Aug;31:297-312 PMID: 14056989
  33. STUDIES ON THYMIDINE KINASE IN CULTURED MOUSE FIBROBLASTS.
    Biochim Biophys Acta. 1965 Jan 11;95:14-22 PMID: 14289021
  34. Independent immunoglobulin class-switch events occurring in a single myeloma cell line.
    Mol Cell Biol. 1985 Apr;5(4):856-68 PMID: 3921826
  35. Simultaneous expression of mu- and gamma-chain mRNA in cloned murine B-lymphoma cell lines.
    EMBO J. 1983;2(2):167-72 PMID: 11894921
  36. Regulation of melanin synthesis in mammalian cells, as studied by somatic hybridization. I. Evidence for negative control.
    J Cell Physiol. 1968 Oct;72(2):115-27 PMID: 4971858
  37. IgG synthesis in hybrid cells from an antibody-producing mouse myeloma and an L cell substrain.
    Nature. 1970 Dec 12;228(5276):1086-7 PMID: 5483162
  38. Expression of tyrosine aminotransferase activity in somatic-cell heterokaryons: evidence for negative control of enzyme expression.
    Proc Natl Acad Sci U S A. 1971 Oct;68(10):2589-93 PMID: 4109409
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.2.936-939.1987
Published
1987-02-00
Pages
936-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365154
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.7.2.936-939.1987 PMC Full Text View journal details
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