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PMID: 3112526 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning and expression of streptococcal pyrogenic exotoxin A and staphylococcal toxic shock syndrome toxin-1 in Bacillus subtilis.

Molecular & general genetics : MGG ·Vol. 208 ·No. 1-2 ·1987-06-00 ·Pages 84-7

Kreiswirth BN, Handley JP, Schlievert PM, Novick RP

Abstract

The genes encoding streptococcal pyrogenic exotoxin type A (SPE A) and staphylococcal toxic shock syndrome toxin-1 (TSST-1) were stably cloned and expressed in Bacillus subtilis. In the non-pathogenic Bacillus background, the recombinant speA clone expressed 32-fold more SPE A than the native streptococcus, and similarly, the recombinant plasmid harboring tst expressed 4-fold more TSST-1 in Bacillus than in the native Staphylococcus aureus. The Bacillus-derived products were secreted into the culture fluid, were resistant to proteolytic degradation and their biological activities mimicked native preparations.

MeSH Terms
Bacillus subtilis/genetics Bacterial Proteins Bacterial Toxins Biological Assay Cloning, Molecular Enterotoxins/genetics Exotoxins/genetics Gene Expression Regulation Genes, Bacterial Genetic Vectors Membrane Proteins Staphylococcus/genetics Streptococcus/genetics Superantigens
Chemicals
Bacterial Proteins Bacterial Toxins Enterotoxins Exotoxins Membrane Proteins SpeA protein, Streptococcus pyogenes Superantigens enterotoxin F, Staphylococcal erythrogenic toxin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kreiswirth B N
Handley J P
Schlievert P M
Novick R P
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22 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00330426
Published
1987-06-00
Pages
84-7
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIAID NIH HHS · AI22159 · United States
External Links
PubMed source DOI: 10.1007/BF00330426
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