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PMID: 3118190 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Plasminogen activator inhibitor type 1 biosynthesis and mRNA level are increased by dexamethasone in human fibrosarcoma cells.

Molecular and cellular biology ·Vol. 7 ·No. 8 ·1987-08-00 ·Pages 3021-5

Andreasen PA, Pyke C, Riccio A, Kristensen P, Nielsen LS, Lund LR, Blasi F, Danø K

Abstract

Dexamethasone increases type 1 plasminogen activator inhibitor (PAI-1) activity released from the human fibrosarcoma cell line HT-1080. We demonstrated that dexamethasone caused about 10-fold increases in the intracellular and extracellular levels of PAI-1 protein, as measured by an enzyme-linked immunosorbent assay, in the rate of PAI-1 biosynthesis, and in the PAI-1 mRNA level. The effects on PAI-1 biosynthesis and mRNA level were detectable within 4 h and were maximal 16 to 24 h after the addition of dexamethasone. Cycloheximide did not inhibit the dexamethasone-induced increases in the capacity of the cells to synthesize PAI-1 and in the PAI-1 mRNA level.

MeSH Terms
Cell Line Cycloheximide/pharmacology Dexamethasone/pharmacology Fibrosarcoma/metabolism Humans Kinetics Molecular Weight RNA, Messenger/drug effects,genetics Tissue Plasminogen Activator/biosynthesis,genetics,isolation & purification
Chemicals
RNA, Messenger Dexamethasone Cycloheximide Tissue Plasminogen Activator
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Andreasen P A
Finsen Laboratory, Rigshospitalet, Copenhagen, Denmark.
Pyke C
Riccio A
Kristensen P
Nielsen L S
Lund L R
Blasi F
Danø K
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.8.3021-3025.1987
Published
1987-08-00
Pages
3021-5
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367928
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.7.8.3021-3025.1987 PMC Full Text View journal details
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