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PMID: 3127381 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Translational attenuation control of ermSF, an inducible resistance determinant encoding rRNA N-methyltransferase from Streptomyces fradiae.

Journal of bacteriology ·Vol. 170 ·No. 4 ·1988-04-00 ·Pages 1800-11

Kamimiya S, Weisblum B

Abstract

An inducible resistance determinant, ermSF, from the tylosin producer Streptomyces fradiae NRRL 2338 has been cloned, sequenced, and shown to confer inducible macrolide-lincosamide-streptogramin B resistance when transferred to Streptomyces griseofuscus NRRL 23916. From mapping studies with S1 nuclease to locate the site of transcription initiation, the ermSF message contains a 385-nucleotide 5' leader sequence upstream from the 960-nucleotide major open reading frame that encodes the resistance determinant. On the basis of the potential secondary structure that the ermSF leader can assume, a translational attenuation model similar to that for ermC is proposed. The model is supported by mutational analysis involving deletions in the proposed attenuator. By analysis with restriction endonucleases, ermSF is indistinguishable from the tlrA gene described by Birmingham et al. (V. A. Birmingham, K. L. Cox, J. L. Larson, S. E. Fishman, C. L. Hershberger, and E. T. Seno, Mol. Gen. Genet. 204:532-539, 1986) which comprises one of at least three genes from S. fradiae that can confer tylosin resistance when subcloned into S. griseofuscus. When tested for inducibility, ermSF appears to be strongly induced by erythromycin, but not by tylosin.

MeSH Terms
Amino Acid Sequence Anti-Bacterial Agents/pharmacology Base Sequence Cloning, Molecular DNA, Bacterial/genetics Drug Resistance, Microbial/genetics Erythromycin/pharmacology Gene Expression Regulation Genes, Bacterial Leucomycins/pharmacology Lincosamides Macrolides Methyltransferases/genetics Molecular Sequence Data Mutation Phenotype Protein Biosynthesis Protein Sorting Signals/genetics Streptomyces/drug effects,enzymology,genetics Transcription, Genetic Virginiamycin/pharmacology
Chemicals
Anti-Bacterial Agents DNA, Bacterial Leucomycins Lincosamides Macrolides Protein Sorting Signals Virginiamycin Erythromycin Methyltransferases rRNA (adenosine-O-2'-)methyltransferase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kamimiya S
Pharmacology Department, University of Wisconsin Medical School, Madison 53706.
Weisblum B
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.4.1800-1811.1988
Published
1988-04-00
Pages
1800-11
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211034
Subset
IM
Grants
NIAID NIH HHS · AI-18283 · United States
NCI NIH HHS · CA-07175 · United States
NCRR NIH HHS · S10-RR01684 · United States
Databases
GENBANK
M19269
External Links
PubMed source DOI: 10.1128/jb.170.4.1800-1811.1988 PMC Full Text View journal details
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