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PMID: 3133550 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Genes activated in the presence of an immunoglobulin enhancer or promoter are negatively regulated by a T-lymphoma cell line.

Molecular and cellular biology ·Vol. 8 ·No. 5 ·1988-05-00 ·Pages 1932-9

Zaller DM, Yu H, Eckhardt LA

Abstract

The tissue-specific expression of immunoglobulin genes can be partially explained by a requirement for activating factors found only in B lymphocytes and their derivatives. However, loss of immunoglobulin expression upon fusion of an immunoglobulin-producing myeloma cell with a T lymphoma cell (BW5147) or fibroblast (L cell) suggests that negatively acting factors also play a role in the tissue specificity of immunoglobulin genes. Expression of a cloned immunoglobulin heavy-chain gene introduced into myeloma cells was suppressed after fusion of the myeloma transformants with BW5147. The presence of either the immunoglobulin heavy-chain enhancer or promoter conferred suppression, under similar conditions, upon a heterologous gene that is normally expressed in both B and T lymphocytes. These immunoglobulin heavy-chain gene control regions, or gene modifications induced by them, are subject to negative control by T-lymphocyte-derived factors.

MeSH Terms
Animals Cell Fusion Enhancer Elements, Genetic Gene Expression Regulation Genes, Immunoglobulin Hybridomas/metabolism,pathology Immunoglobulin Heavy Chains/biosynthesis,genetics Lymphoma/pathology Mice Mice, Inbred AKR Mice, Inbred BALB C Promoter Regions, Genetic Recombinant Proteins/biosynthesis,genetics T-Lymphocytes/metabolism Tumor Cells, Cultured/metabolism
Chemicals
Immunoglobulin Heavy Chains Recombinant Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zaller D M
Department of Biological Sciences, Columbia University, New York, New York 10027.
Yu H
Eckhardt L A
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.5.1932-1939.1988
Published
1988-05-00
Pages
1932-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363371
Subset
IM
Grants
NIAID NIH HHS · AI21578 · United States
PHS HHS · M0721609 · United States
External Links
PubMed source DOI: 10.1128/mcb.8.5.1932-1939.1988 PMC Full Text View journal details
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