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PMID: 3133637 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation and structural analysis of a ribosomal protein gene in D.melanogaster.

Nucleic acids research ·Vol. 16 ·No. 11 ·1988-06-10 ·Pages 4915-26

Rafti F, Gargiulo G, Manzi A, Malva C, Grossi G, Andone S, Graziani F

Abstract

By using the cDNA clone containing the sequence for the L1 ribosomal protein gene of Xenopus laevis as probe (1), we have isolated positive phages from a Drosophila melanogaster genomic library. The Drosophila genomic fragment, which gives the hybridization signal with the Xenopus cDNA, was sequenced: a region of 369 bp is 70% homologous to the sequence of X. laevis L1 cDNA. The gene was localized in situ at position 98AB of the right arm of the third polytene chromosome. By S1 mapping and heteroduplex analysis we have found that the gene is interrupted by three introns. A Drosophila cDNA embryonic library was screened and three cDNA clones were isolated (900, 1400 and 1500 nt long). By Northern analysis the cDNAs identify a 1400nt transcript present at every stage of development. By the features described, the clones we have isolated identify the Drosophila rp gene homologous to the L1 rp gene of Xenopus and could code for the L1 ribosomal protein described in D. melanogaster.

MeSH Terms
Amino Acid Sequence Animals Bacterial Proteins/genetics Base Sequence DNA/genetics DNA, Recombinant Drosophila melanogaster/genetics Genes Molecular Sequence Data Ribosomal Proteins/genetics
Chemicals
Bacterial Proteins DNA, Recombinant Ribosomal Proteins ribosomal protein L1 DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Rafti F
Istituto Internazionale di Genetica e Biofiscia, CNR, Naples, Italy.
Gargiulo G
Manzi A
Malva C
Grossi G
Andone S
Graziani F
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.11.4915
Published
1988-06-10
Pages
4915-26
Language
English
Region
England
NLM ID
0411011
PMCID
PMC336706
Subset
IM
Databases
GENBANK
X06881
External Links
PubMed source DOI: 10.1093/nar/16.11.4915 PMC Full Text View journal details
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