The intron requirement for immunoglobulin gene expression is dependent upon the promoter.

Nucleic acids research ·Vol. 16 ·No. 14B ·1988-07-25 ·Pages 6713-24

Neuberger MS, Williams GT

Abstract

Transfection assays were used to assess the need for an intron in order to obtain expression of cytoplasmic immunoglobulin mu mRNA. An intron is required when transcription is driven by an immunoglobulin promoter/enhancer combination, although this requirement is not specific for a particular intron. However, this need for an intron is dependent upon the promoter used. Whilst an intron is required in the case of immunoglobulin or beta-globin promoters, it is not in the case of cytomegalovirus or heat-shock promoters. The data point to a connection between the promoter and RNA processing or export.

MeSH Terms

Enhancer Elements, Genetic Gene Expression Regulation Genes, Immunoglobulin Humans Immunoglobulin mu-Chains/genetics Introns Promoter Regions, Genetic RNA Processing, Post-Transcriptional Transcription, Genetic

Chemicals

Immunoglobulin mu-Chains

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Neuberger M S

Medical Research Council Laboratory of Molecular Biology, Cambridge, UK.

Williams G T

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/16.14.6713

Published

1988-07-25

Pages

6713-24

Language

English

Region

England

NLM ID

0411011

PMCID

PMC338327

Subset

External Links

PubMed source DOI: 10.1093/nar/16.14.6713 PMC Full Text View journal details

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The intron requirement for immunoglobulin gene expression is dependent upon the promoter.

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