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PMID: 3119328 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Polymeric immunoglobulin M is secreted by transfectants of non-lymphoid cells in the absence of immunoglobulin J chain.

The EMBO journal ·Vol. 6 ·No. 9 ·1987-09-00 ·Pages 2753-8

Cattaneo A, Neuberger MS

Abstract

Plasmids were constructed in which expression of genes encoding the heavy and light chains of a hapten-specific IgM antibody is under control of a heat shock promoter. Glioma, phaeochromocytoma and other non-lymphoid cell lines transfected with the plasmids were able to process and secrete immunoglobulin following heat induction. The glioma transfectants were studied in detail and were shown to secrete polymeric IgM in a yield similar to that obtained with a plasmacytoma. However, the glioma IgM was not associated with J chain and was largely composed of pentamers and hexamers. Thus, neither J chain nor other lymphoid-specific proteins are required for assembly and secretion of polymeric IgM although the absence of J chain may encourage hexamer formation.

MeSH Terms
Animals Cell Line Genes, Immunoglobulin Immunoglobulin J-Chains/genetics Immunoglobulin M/analysis,genetics Lymphocytes/immunology Macromolecular Substances Plasmids
Chemicals
Immunoglobulin J-Chains Immunoglobulin M Macromolecular Substances
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cattaneo A
Medical Research Council Laboratory of Molecular Biology, Cambridge, UK.
Neuberger M S
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-09-00
Pages
2753-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553699
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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