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PMID: 6374653 Published · ppublish English Journal Article

Generation of antibody activity from immunoglobulin polypeptide chains produced in Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 11 ·1984-06-00 ·Pages 3273-7

Cabilly S, Riggs AD, Pande H, Shively JE, Holmes WE, Rey M, Perry LJ, Wetzel R, Heyneker HL

Abstract

Plasmids have been constructed that direct the synthesis in Escherichia coli of heavy chains and/or light chains of an anti-carcinoembryonic antigen (CEA) antibody. Another plasmid was constructed for expression of a truncated form of heavy chain (Fd' fragment) in E. coli. Functional CEA-binding activity was obtained by in vitro reconstitution in E. coli extracts of heavy chain or Fd' fragment mixed with extracts containing light chain.

MeSH Terms
Amino Acid Sequence Antibodies, Monoclonal/biosynthesis Antibodies, Neoplasm/biosynthesis,genetics Antibody Specificity Carcinoembryonic Antigen/immunology Cloning, Molecular/methods DNA/genetics DNA, Recombinant Escherichia coli/genetics Macromolecular Substances
Chemicals
Antibodies, Monoclonal Antibodies, Neoplasm Carcinoembryonic Antigen DNA, Recombinant Macromolecular Substances DNA
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Cabilly S
Riggs A D
Pande H
Shively J E
Holmes W E
Rey M
Perry L J
Wetzel R
Heyneker H L
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.11.3273
Published
1984-06-00
Pages
3273-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345489
Subset
IM
Databases
GENBANK
K02152, K02156
External Links
PubMed source DOI: 10.1073/pnas.81.11.3273 PMC Full Text View journal details
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