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PMID: 3437897 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Introns are inconsequential to efficient formation of cellular thymidine kinase mRNA in mouse L cells.

Molecular and cellular biology ·Vol. 7 ·No. 12 ·1987-12-00 ·Pages 4576-81

Gross MK, Kainz MS, Merrill GF

Abstract

TK mRNA levels were determined in mouse L cells transformed with intron deletion mutations of the chicken TK gene. Whether normalized per cell, per integrated gene, or per internal control signal, intron deletion did not diminish the efficiency of TK mRNA formation in transformed L cells. The results demonstrated that introns are not required for efficient biogenesis of cellular mRNA in transformed mouse L cells.

MeSH Terms
Animals Chickens Introns L Cells Mice Mutation Nucleic Acid Hybridization RNA Splicing RNA, Messenger/biosynthesis Ribonucleases Thymidine Kinase/genetics Transcription, Genetic Transformation, Genetic
Chemicals
RNA, Messenger Thymidine Kinase Ribonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gross M K
Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331.
Kainz M S
Merrill G F
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26 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.12.4576-4581.1987
Published
1987-12-00
Pages
4576-81
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368147
Subset
IM
Grants
NIA NIH HHS · AG-00334 · United States
NIGMS NIH HHS · GM-34432 · United States
External Links
PubMed source DOI: 10.1128/mcb.7.12.4576-4581.1987 PMC Full Text View journal details
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