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PMID: 3822814 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cell cycle regulated synthesis of stable mouse thymidine kinase mRNA is mediated by a sequence within the cDNA.

Nucleic acids research ·Vol. 15 ·No. 2 ·1987-01-26 ·Pages 741-52

Hofbauer R, Müllner E, Seiser C, Wintersberger E

Abstract

The cDNA for mouse thymidine kinase (TK) was isolated from a cDNA library in lambda-gt11 and sequenced. It was used as a probe to follow the time course of TK mRNA expression in growth stimulated mouse fibroblasts. Linked to the HSV-TK promoter the cDNA was able to transform LTK-cells to the TK+ phenotype. The transformed cells expressed the TK mRNA and enzyme activity in a growth dependent fashion suggesting that the regulatory element is localized on the cDNA.

MeSH Terms
Animals Base Sequence Cell Cycle DNA/metabolism Embryo, Mammalian L Cells/enzymology Mice RNA, Messenger/biosynthesis,genetics Thymidine Kinase/deficiency,genetics
Chemicals
RNA, Messenger DNA Thymidine Kinase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hofbauer R
Müllner E
Seiser C
Wintersberger E
References (25)
25 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.2.741
Published
1987-01-26
Pages
741-52
Language
English
Region
England
NLM ID
0411011
PMCID
PMC340464
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/15.2.741 PMC Full Text View journal details
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